Identification of two Melan-A CD4+ T cell epitopes presented by frequently expressed MHC class II alleles Journal Article
| Authors: | Godefroy, E.; Scotto, L.; Souleimanian, N. E.; Ritter, G.; Old, L. J.; Jotereau, F.; Valmori, D.; Ayyoub, M. |
| Article Title: | Identification of two Melan-A CD4+ T cell epitopes presented by frequently expressed MHC class II alleles |
| Abstract: | Because of its expression pattern restricted to cells of the melanocytic lineage and to melanoma cells, Melan-A is an important target of immunotherapeutic approaches for the treatment of melanoma. Identification of Melan-A derived sequences recognized by specific T cells is therefore of great interest for the development of these therapeutic strategies. Using circulating CD4+ T cells from healthy donors, we identified two Melan-A-derived CD4+ T cell epitopes mapping to the 1-20 and 91-110 regions of the protein and restricted by HLA-DR11 and HLA-DR52 molecules, respectively. CD4+ T cells specific for the identified epitopes were able to recognize the native antigen when endogenously expressed by antigen presenting cells and tumor cells. In addition, CD4+ T cells specific for Melan-A 91-110 recognized the epitope after exogenous processing and presentation of Melan-A recombinant protein. Identification of these epitopes will be instrumental for the evaluation of the immune response to Melan-A in cancer patients. © 2006 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; unclassified drug; human cell; allele; melanoma; gene expression; neoplasm proteins; alleles; cell line, tumor; cell specificity; antigen presentation; antigens, neoplasm; antigen specificity; hla dr antigen; major histocompatibility antigen class 2; antigen recognition; cd4+ t lymphocyte; cd4-positive t-lymphocytes; epitope mapping; hla-dr antigens; cell line, transformed; tumor immunity; melan a; histocompatibility antigens class ii; epitopes, t-lymphocyte; clone cells; antigen presenting cell; vaccine; normal human; major histocompatibility complex restriction; peptide mapping; cd4+ t cells; melan-a; hla dr11 antigen; hla dr52 antigen; hla-dr5 antigen |
| Journal Title: | Clinical Immunology |
| Volume: | 121 |
| Issue: | 1 |
| ISSN: | 1521-6616 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2006-10-01 |
| Start Page: | 54 |
| End Page: | 62 |
| Language: | English |
| DOI: | 10.1016/j.clim.2006.05.007 |
| PUBMED: | 16814609 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 16" - "Export Date: 4 June 2012" - "CODEN: CLIIF" - "Source: Scopus" |
