Reactivity of autologous CD4(+) T lymphocytes against human melanoma: Evidence for a shared melanoma antigen presented by HLA-DR15 Journal Article
| Authors: | Takahashi, T.; Chapman, P. B.; Yang, S. Y.; Hara, I.; Vijayasaradhi, S.; Houghton, A. N. |
| Article Title: | Reactivity of autologous CD4(+) T lymphocytes against human melanoma: Evidence for a shared melanoma antigen presented by HLA-DR15 |
| Abstract: | Reactivity of CD8+ T lymphocytes against human melanoma has been extensively characterized, but little is known about melanoma Ags recognized by CD4+ lymphocytes. We have identified CD4+ CTL that recognize shared melanoma Ag(s) expressed by autologous melanoma cells and a subset of allogeneic melanomas. The same Ag(s) was shared by autologous and positive allogeneic melanomas by cross-blocking experiments. Cytotoxicity was directed against epitopes presented by HLA-DR on target melanoma cells, and allelic typing revealed that cytotoxicity was restricted through HLA-DR15. These CD4+ T cells released IFN-γ, IL-4, and TNF-α, but not IL-2, in response to HLA-DR15+ target cells. CD4+ T cells did not lyse DR15+ nonmelanoma cell types, including melanocytes or fibroblasts (induced to express HLA-DR by IFN-γ). Thus, by cytotoxicity assays, shared Ags were only recognized on melanoma cells but not on normal melanocytes. In summary, this analysis shows that melanoma cells share an Ag that is presented by HLA-DR15. |
| Keywords: | controlled study; human cell; flow cytometry; cd3 antigen; cd8 antigen; t lymphocyte; phenotype; allele; interleukin 2; melanoma; interleukin 4; cell line; tumor cells, cultured; enzyme linked immunosorbent assay; cd16 antigen; antigen presentation; cytokines; antibodies, monoclonal; molecular sequence data; tumor necrosis factor alpha; antigens, neoplasm; gamma interferon; hla antigen class 2; hla dr antigen; major histocompatibility antigen class 2; cd4-positive t-lymphocytes; hla-dr antigens; epitope; t-lymphocytes, cytotoxic; base sequence; melanoma antigen; cd4 antigen; ganglioside gd3; cell mediated cytotoxicity; interleukin 2 receptor; cytotoxicity tests, immunologic; binding, competitive; cd56 antigen; beta1 integrin; oligonucleotide probes; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Immunology |
| Volume: | 154 |
| Issue: | 2 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1995-01-15 |
| Start Page: | 772 |
| End Page: | 779 |
| Language: | English |
| PUBMED: | 7814883 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 28 August 2018 -- Source: Scopus |
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