UV irradiation induces a postreplication DNA damage checkpoint Journal Article
| Authors: | Callegari, A. J.; Kelly, T. J. |
| Article Title: | UV irradiation induces a postreplication DNA damage checkpoint |
| Abstract: | Eukaryotic cells irradiated with high doses of UV exhibit cell-cycle responses referred to as G1/S, intraS, and G2/M checkpoints. After a moderate UV dose that approximates sunlight exposure and is lethal to fission yeast checkpoint mutants, we found unexpectedly that these cell-cycle responses do not occur. Instead, cells at all stages of the cell cycle carry lesions into S phase and delay cell-cycle progression for hours after the completion of bulk DNA synthesis. Both DNA replication and the checkpoint kinase, Chk1, are required to generate this cell-cycle response. UV-irradiation of Δchk1 cells causes chromosome damage and loss of viability only after cells have replicated irradiated DNA and entered mitosis. These data suggest that an important physiological role of the cell-cycle response to UV is to provide time for postreplication repair. © 2006 by The National Academy of Sciences of the USA. |
| Keywords: | mutation; nonhuman; radiation dose; dna replication; dna synthesis; ultraviolet radiation; mitosis; cell viability; dna damage; cell cycle; cell cycle s phase; protein kinases; eukaryota; excision repair; cell cycle g2 phase; cell cycle m phase; yeast; eukaryotic cell; ultraviolet rays; checkpoint kinase 1; cell cycle g1 phase; schizosaccharomyces; schizosaccharomyces pombe; chk1; dna, fungal; chromosome damage; nucleotide excision repair |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 103 |
| Issue: | 43 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2006-10-24 |
| Start Page: | 15877 |
| End Page: | 15882 |
| Language: | English |
| DOI: | 10.1073/pnas.0607343103 |
| PUBMED: | 17043220 |
| PROVIDER: | scopus |
| PMCID: | PMC1613229 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 19" - "Export Date: 4 June 2012" - "CODEN: PNASA" - "Source: Scopus" |
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