Extensive DNA damage-induced sumoylation contributes to replication and repair and acts in addition to the Mec1 checkpoint Journal Article
| Authors: | Cremona, C. A.; Sarangi, P.; Yang, Y.; Hang, L. E.; Rahman, S.; Zhao, X. |
| Article Title: | Extensive DNA damage-induced sumoylation contributes to replication and repair and acts in addition to the Mec1 checkpoint |
| Abstract: | The cellular response to DNA damage employs multiple dynamic protein modifications to exert rapid and adaptable effects. Substantial work has detailed the roles of canonical checkpoint-mediated phosphorylation in this program. Recent studies have also implicated sumoylation in the DNA damage response; however, a systematic view of the contribution of sumoylation to replication and repair and its interplay with checkpoints is lacking. Here, using a biochemical screen in yeast, we establish that DNA damage-induced sumoylation occurs on a large scale. We identify MRX (Mre11-Rad50-Xrs2) as a positive regulator of this induction for a subset of repair targets. In addition, we find that defective sumoylation results in failure to complete replication of a damaged genome and impaired DNA end processing, highlighting the importance of the SUMO-mediated response in genome integrity. We also show that DNA damage-induced sumoylation does not require Mec1 checkpoint signaling, and the presence of both enables optimal DNA damage resistance. © 2012 Elsevier Inc. |
| Keywords: | signal transduction; controlled study; dna-binding proteins; nonhuman; dna replication; dna damage; dna repair; phosphorylation; protein processing, post-translational; saccharomyces cerevisiae; protein-serine-threonine kinases; genomic instability; intracellular signaling peptides and proteins; dna repair enzymes; multiprotein complexes; yeast; saccharomyces cerevisiae proteins; sumoylation; biochemistry; microbial viability; genome, fungal; dna damage checkpoint; gene knockout techniques; cell cycle checkpoints |
| Journal Title: | Molecular Cell |
| Volume: | 45 |
| Issue: | 3 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2012-02-10 |
| Start Page: | 422 |
| End Page: | 432 |
| Language: | English |
| DOI: | 10.1016/j.molcel.2011.11.028 |
| PROVIDER: | scopus |
| PUBMED: | 22285753 |
| PMCID: | PMC3340930 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 March 2012" - "CODEN: MOCEF" - "Source: Scopus" |
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