DNA break-induced sumoylation is enabled by collaboration between a SUMO ligase and the ssDNA-binding complex RPA Journal Article
| Authors: | Chung, I.; Zhao, X. |
| Article Title: | DNA break-induced sumoylation is enabled by collaboration between a SUMO ligase and the ssDNA-binding complex RPA |
| Abstract: | Upon genome damage, large-scale protein sumoylation occurs fromyeast to humans to promoteDNArepair. Currently, the underlying mechanism is largely unknown. Here we show that, upon DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance. Both RPA and nuclease-generated ssDNA promote Siz2-mediated sumoylation. Mechanistically, the conserved Siz2 interaction with RPA enables Siz2 localization to damage sites. These findings provide a molecular basis for recruitingSUMOligases to the vicinity of their substrates to induce sumoylation upon DNA damage. © 2015 Chung and Zhao. |
| Keywords: | controlled study; unclassified drug; protein localization; dna damage; homologous recombination; protein depletion; protein protein interaction; protein; dna strand breakage; amino acid sequence; dna damage response; nuclease; saccharomycetales; replication factor a; single stranded dna; dna binding; sumo protein; sumoylation; phleomycin; priority journal; article; protein siz2 |
| Journal Title: | Genes and Development |
| Volume: | 29 |
| Issue: | 15 |
| ISSN: | 0890-9369 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2015-08-01 |
| Start Page: | 1593 |
| End Page: | 1598 |
| Language: | English |
| DOI: | 10.1101/gad.265058.115 |
| PROVIDER: | scopus |
| PMCID: | PMC4536307 |
| PUBMED: | 26253534 |
| DOI/URL: | |
| Notes: | Export Date: 2 September 2015 -- Source: Scopus |
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