Cooperation of sumoylated chromosomal proteins in rDNA maintenance Journal Article
| Authors: | Takahashi, Y.; Dulev, S.; Liu, X.; Hiller, N. J.; Zhao, X.; Strunnikov, A. |
| Article Title: | Cooperation of sumoylated chromosomal proteins in rDNA maintenance |
| Abstract: | SUMO is a posttranslational modifier that can modulate protein activities, interactions, and localizations. As the GFP-Smt3p fusion protein has a preference for subnucleolar localization, especially when deconjugation is impaired, the nucleolar role of SUMO can be the key to its biological functions. Using conditional triple SUMO E3 mutants, we show that defects in sumoylation impair rDNA maintenance, i.e., the rDNA segregation is defective and the rDNA copy number decreases in these mutants. Upon characterization of sumoylated proteins involved in rDNA maintenance, we established that Top1p and Top2p, which are sumoylated by Siz1p/Siz2p, most likely collaborate with substrates of Mms21p to maintain rDNA integrity. Cohesin and condensin subunits, which both play important roles in rDNA stability and structures, are potential substrates of Mms21, as their sumoylation depends on Mms21p, but not Siz1p and Siz2p. In addition, binding of cohesin and condensin to rDNA is altered in the mms21-CH E3-deficient mutant. |
| Keywords: | gene mutation; genetics; mutation; nonhuman; mutant protein; cohesin; nonhistone protein; metabolism; chromosomal proteins, non-histone; nuclear protein; ubiquitin protein ligase; protein binding; nuclear proteins; molecular cloning; protein processing; protein processing, post-translational; protein purification; saccharomyces cerevisiae; gene interaction; gene control; saccharomyces cerevisiae proteins; protein subunit; saccharomyces cerevisiae protein; sumo protein; sumoylation; sumo-1 protein; condensin; chromosome protein; ubiquitin-protein ligases; small ubiquitin-related modifier proteins; dna topoisomerase; fungal gene; nucleolus; dna, fungal; sumo 1 protein; fungal dna; dna, ribosomal; genes, fungal; ribosome dna; dna topoisomerases; mms21 protein, s cerevisiae; siz1 protein, s cerevisiae; siz2 protein, s cerevisiae; gene segregation |
| Journal Title: | PLoS Genetics |
| Volume: | 4 |
| Issue: | 10 |
| ISSN: | 1553-7390 |
| Publisher: | Public Library of Science |
| Date Published: | 2008-10-01 |
| Start Page: | e1000215 |
| Language: | English |
| DOI: | 10.1371/journal.pgen.1000215 |
| PUBMED: | 18846224 |
| PROVIDER: | scopus |
| PMCID: | PMC2563031 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 7" - "Export Date: 17 November 2011" - "Source: Scopus" |
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