Rad52 SUMOylation affects the efficiency of the DNA repair Journal Article

Authors: Altmannova, V.; Eckert-Boulet, N.; Arneric, M.; Kolesar, P.; Chaloupkova, R.; Damborsky, J.; Sung, P.; Zhao, X.; Lisby, M.; Krejci, L.
Article Title: Rad52 SUMOylation affects the efficiency of the DNA repair
Abstract: Homologous recombination (HR) plays a vital role in DNA metabolic processes including meiosis, DNA repair, DNA replication and rDNA homeostasis. HR defects can lead to pathological outcomes, including genetic diseases and cancer. Recent studies suggest that the post-translational modification by the small ubiquitin-like modifier (SUMO) protein plays an important role in mitotic and meiotic recombination. However, the precise role of SUMOylation during recombination is still unclear. Here, we characterize the effect of SUMOylation on the biochemical properties of the Saccharomyces cerevisiae recombination mediator protein Rad52. Interestingly, Rad52 SUMOylation is enhanced by single-stranded DNA, and we show that SUMOylation of Rad52 also inhibits its DNA binding and annealing activities. The biochemical effects of SUMO modification in vitro are accompanied by a shorter duration of spontaneous Rad52 foci in vivo and a shift in spontaneous mitotic recombination from single-strand annealing to gene conversion events in the SUMO-deficient Rad52 mutants. Taken together, our results highlight the importance of Rad52 SUMOylation as part of a 'quality control' mechanism regulating the efficiency of recombination and DNA repair. © The Author(s) 2010. Published by Oxford University Press.
Keywords: controlled study; protein expression; nonhuman; protein domain; quality control; homologous recombination; dna repair; protein dna binding; protein protein interaction; in vivo study; in vitro study; enzyme activity; gene conversion; protein purification; saccharomyces cerevisiae; recombination, genetic; nucleotide sequence; protein structure, tertiary; saccharomyces cerevisiae proteins; single stranded dna; dna, single-stranded; sumoylation; sumo-1 protein; lysine; replication protein a; protein modification; rad51 recombinase; rad52 protein; circular dichroism; fungal strain; mitotic recombination; rad52 dna repair and recombination protein
Journal Title: Nucleic Acids Research
Volume: 38
Issue: 14
ISSN: 0305-1048
Publisher: Oxford University Press  
Date Published: 2010-08-01
Start Page: 4708
End Page: 4721
Language: English
DOI: 10.1093/nar/gkq195
PUBMED: 20371517
PROVIDER: scopus
PMCID: PMC2919706
Notes: --- - "Cited By (since 1996): 1" - "Export Date: 20 April 2011" - "Art. No.: gkq195" - "CODEN: NARHA" - "Source: Scopus"
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  1. Xiaolan Zhao
    49 Zhao