Generation of prefrontal cortex neurons from human pluripotent stem cells under chemically defined conditions Journal Article

  


Authors: Cederquist, G. Y.; Oberst, P.; Chang, X.; Tchieu, J.; Studer, L.
Article Title: Generation of prefrontal cortex neurons from human pluripotent stem cells under chemically defined conditions
Abstract: Human pluripotent stem cells (hPSCs) have the potential to differentiate into all human somatic cell types allowing for an experimental platform to access otherwise inaccessible tissues through directed differentiation protocols. Access to tissue is especially critical for neurobiology where functional human tissue is rare. The prefrontal cortex is an evolutionarily expanded addition to the cerebral cortex, associated with higher order cognitive function. Here, we present a method to generate neural progenitors and post-mitotic neurons with a prefrontal cortical identity through the directed differentiation of hPSCs under defined conditions. Neural induction is achieved by inhibiting TGF beta and BMP signaling (termed dual-SMAD inhibition) and neural progenitors are subsequently patterned toward the prefrontal cortex lineage using recombinant FGF8. Cells generated using this protocol open possibilities to study the unique developmental and synaptic properties of the prefrontal cortex, as well as to understand its selective vulnerability in a number of human brain disorders. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.
Keywords: signal transduction; controlled study; human tissue; human cell; mitosis; quality control; cytology; metabolism; cells, cultured; somatic cell; transforming growth factor beta; brain cortex; neural stem cell; cell differentiation; neurons; drug effect; genetic recombination; cell culture; cell culture techniques; pluripotent stem cell; pluripotent stem cells; neural stem cells; nerve cell differentiation; nervous system development; neurogenesis; nerve cell; vitronectin; fibroblast growth factor 8; directed differentiation; prefrontal cortex; neurodevelopment; procedures; human pluripotent stem cells; monolayer culture; bmp signaling; induced pluripotent stem cell; humans; human; occipital cortex; cell culture technique; human disease modeling
Journal Title: Methods in Molecular Biology
Volume: 2910
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2025-01-01
Start Page: 37
End Page: 51
Language: English
DOI: 10.1007/978-1-0716-4446-1_3
PROVIDER: scopus
PUBMED: 40220092
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-105002758828&doi=10.1007%2f978-1-0716-4446-1_3&partnerID=40&md5=ebd3382248451575ea54c9e27d61c271
Notes: Book Chapter in "Synapse Development: Methods and Protocols. 2nd ed." (ISBN: 978-1-0716-4445-4) -- Source: Scopus
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MSK Authors
  1. Lorenz Studer
    220 Studer
  2. Gustav Y Cederquist
    35 Cederquist
  3. Polina Oberst
    3 Oberst
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