Electrolysis products, reactive oxygen species and ATP loss contribute to cell death following irreversible electroporation with microsecond-long pulsed electric fields Journal Article
| Authors: | Rajagopalan, N. R.; Munawar, T.; Sheehan, M. C.; Fujimori, M.; Vista, W. R.; Wimmer, T.; Gutta, N. B.; Solomon, S. B.; Srimathveeravalli, G. |
| Article Title: | Electrolysis products, reactive oxygen species and ATP loss contribute to cell death following irreversible electroporation with microsecond-long pulsed electric fields |
| Abstract: | Membrane permeabilization and thermal injury are the major cause of cell death during irreversible electroporation (IRE) performed using high electric field strength (EFS) and small number of pulses. In this study, we explored cell death under conditions of reduced EFS and prolonged pulse application, identifying the contributions of electrolysis, reactive oxygen species (ROS) and ATP loss. We performed ablations with conventional high-voltage low pulse (HV-LP) and low-voltage high pulse (LV-HP) conditions in a 3D tumor mimic, finding equivalent ablation volumes when using 2000 V/cm 90 pulses or 1000 V/cm 900 pulses respectively. These results were confirmed by performing ablations in swine liver. In LV-HP treatment, ablation volume was found to increase proportionally with pulse numbers, without the substantial temperature increase seen with HV-LP parameters. Peri-electrode pH changes, ATP loss and ROS production were seen in both conditions, but LV-HP treatments were more sensitive to blocking of these forms of cell injury. Increases in current drawn during HV-LP was not observed during LV-HP condition where the total ablation volume correlated to the charge delivered into the tissue which was greater than HV-LP treatment. LV-HP treatment provides a new paradigm in using pulsed electric fields for tissue ablation with clinically relevant volumes. © 2023 Elsevier B.V. |
| Keywords: | human cell; histopathology; nonhuman; animal tissue; cell death; oxygen; animal experiment; animal model; ph; in vitro study; liver; collagen; reactive oxygen species; reactive oxygen metabolite; electroporation; temperature; adenosine triphosphate; computer model; temperature measurement; electric potential; tissue; pig; ablation; irreversible electroporation; electric field; thermal conductivity; finite element analysis; hydrogel; electric conductivity; electric fields; low voltages; electric current; human; article; condition; membrane permeabilization; yorkshire pig; conductance; pulsed electric field; huh-7 cell line; electric losses; electrolysis; high electric field strengths; high-voltages; pulse condition; thermal injuries; reversible electroporation |
| Journal Title: | Bioelectrochemistry |
| Volume: | 155 |
| ISSN: | 1567-5394 |
| Publisher: | Elsevier BV |
| Date Published: | 2024-02-01 |
| Start Page: | 108579 |
| Language: | English |
| DOI: | 10.1016/j.bioelechem.2023.108579 |
| PROVIDER: | scopus |
| PUBMED: | 37769509 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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