The delayed effects of irreversible electroporation ablation on nerves Journal Article
| Authors: | Schoellnast, H.; Monette, S.; Ezell, P. C.; Maybody, M.; Erinjeri, J. P.; Stubblefield, M. D.; Single, G.; Solomon, S. B. |
| Article Title: | The delayed effects of irreversible electroporation ablation on nerves |
| Abstract: | Objective: To evaluate the delayed effects of irreversible electroporation (IRE) ablation on nerves. Methods: The study was approved by the institutional animal care and use committee. CT-guided IRE-ablation (electric field per distance, 1,500 V/cm; pulse length, 70 μs; number of pulses, 90) of 6 sciatic nerves was performed in 6 pigs that were euthanized 2 months after ablation. The sciatic nerves were harvested immediately after euthanasia for histopathological evaluation. Sections from selected specimens were stained with haematoxylin and eosin (H&E), Masson's trichrome (MT) method for collagen, and immunohistochemistry was performed for S100 and neurofilaments (markers for Schwann cells and axons, respectively). Results: All nerves showed a preserved endoneural architecture and presence of numerous small calibre axons associated with Schwann cell hyperplasia, consistent with axonal regeneration. A fibrous scar was observed in the adjacent muscle tissue, confirming ablation at the site examined. Conclusion: After IRE-ablation of nerves, the preservation of the architecture of the endoneurium and the proliferation of Schwann cells may enable axonal regeneration as demonstrated after 2 months in this study. Key Points: • Irreversible electroporation (IRE) offers promise for non-thermal tumour ablation. • Preservation of endoneural architecture and proliferation of Schwann cells follow IRE-ablation. • Preservation of architecture and proliferation of Schwann cells may enable axonal regeneration. • Despite morphological regeneration, nerve function remains variable after 2 months. © 2012 European Society of Radiology. |
| Keywords: | immunohistochemistry; controlled study; histopathology; nonhuman; cell proliferation; animal cell; animal tissue; animal experiment; nerve fiber; clinical evaluation; collagen; electroporation; protein s 100; eosin; hematoxylin; cell regeneration; schwann cell; radiofrequency ablation; nerve regeneration; cell hyperplasia; sciatic nerve; euthanasia; neurofilament; irreversible electroporation; ct-guidance; experimental pig; muscle tissue; nerve conduction; athermal ablation; animal study; endoneurium |
| Journal Title: | European Radiology |
| Volume: | 23 |
| Issue: | 2 |
| ISSN: | 0938-7994 |
| Publisher: | Springer |
| Date Published: | 2013-02-01 |
| Start Page: | 375 |
| End Page: | 380 |
| Language: | English |
| DOI: | 10.1007/s00330-012-2610-3 |
| PROVIDER: | scopus |
| PUBMED: | 23011210 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 1 July 2013" - "CODEN: EURAE" - "Source: Scopus" |
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