Normal skin on ex vivo confocal microscopy: Patterns and histopathologic correlation Book Section
| Authors: | Sendín-Martín, M.; Harris, U.; Farabi, B.; Moronta, M.; Rossi, A.; Lee, E.; Chen, C. S. J.; Nehal, K.; Pulitzer, M.; Jain, M. |
| Editors: | Jain, M.; Rossi, A.; Nehal, K.; Sendín-Martín, M. |
| Article/Chapter Title: | Normal skin on ex vivo confocal microscopy: Patterns and histopathologic correlation |
| Abstract: | In this chapter, we will describe the morphological features of normal layers of skin and its appendages using ex vivo confocal microscopy (EVCM). EVCM is now being integrated during Mohs surgery for margin assessment of the residual skin cancers, such as basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). However, to identify skin cancers it is quintessential to first learn the features of normal skin on EVCME. Using this device, fresh or frozen tissues stained with an acridine orange dye (a fluorescent nuclear dye that enhances visualization of cell nuclei) can be rapidly imaged in reflectance mode (RCM; Reflectance confocal microscopy) or in fluorescence mode (FCM; Fluorescence confocal microscopy), separately or in a combined RCM and FCM mode. The nuclear signal originating from the fluorescent channel is digitally converted to purple colour, resembling hematoxylin-stain on conventional H&E images. The reflectance signal, originating from cell cytoplasm and collagen, is digitally converted to pink colour, resembling eosin-stain on conventional H&E images. Thus, the resultant combined fluorescent and reflectance signal creates a digital H&E (DHE: Digital H&E), purple and pink-coloured images. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. |
| Keywords: | epidermis; dermis; fluorescence confocal microscopy; ex vivo confocal microscopy; digital h&e; adnexal structures; hypodermis; skin layers |
| Book Title: | Cutaneous Atlas of Ex Vivo Confocal Microscopy |
| ISBN: | 978-3-030-89315-6 |
| Publisher: | Springer Nature |
| Publication Place: | Cham, Switzerland |
| Date Published: | 2022-01-01 |
| Start Page: | 29 |
| End Page: | 50 |
| Language: | English |
| DOI: | 10.1007/978-3-030-89316-3_4 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Book Chapter: 4 -- Located in "Part II: Normal Skin" -- Source: Scopus |
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