| Abstract: |
Confocal microscopy (CM) was introduced in dermatology in the last decade. It provides a new rapid imaging approach for evaluation of neoplastic and non-neoplastic lesions, enabling bedside pathology. Ex vivoconfocal microscopy (EVCM) acquires images in two modes: reflectance confocal microscopy (RCM) and fluorescence confocal microscopy (FCM). RCM (grayscale) and FCM (grayscale or green scale) images can be visualized separately or in a combined (RCM + FCM) digitally pseudo- color purple and pink images also called digital H&E (DHE) mode as it resembles conventional hematoxylin-eosin (H&E)-stained images. The DHE image is created by combining signal from FCM (nuclear signal) channel which is digitally converted to purple color (similar to hematoxylin stain) and signal from RCM (cytoplasmic and collagen signal) which is digitally converted to pink color (similar to eosin stain). For information about the principles of ECM device, please refer to Chap. 2. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022. |
