A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease Journal Article
| Authors: | Brandstadter, J. D.; De Martin, A.; Lϋtge, M.; Ferreira, A.; Gaudette, B. T.; Stanossek, Y.; Wang, S.; Gonzalez, M. V.; Camiolo, E.; Wertheim, G.; Austin, B.; Allman, D.; Bagg, A.; Lim, M. S.; Fajgenbaum, D. C.; Aster, J. C.; Ludewig, B.; Maillard, I. |
| Article Title: | A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease |
| Abstract: | Nonhematopoietic lymph node stromal cells (LNSCs) regulate lymphocyte trafficking, survival, and function for key roles in host defense, autoimmunity, alloimmunity, and lymphoproliferative disorders. However, the study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease. Using human tonsils and lymph nodes (LN), lymphoid tissue fragments were cryopreserved for subsequent enzymatic digestion and recovery of viable nonhematopoietic cells. Flow cytometry and single-cell transcriptomics identified comparable proportions of LN stromal cell types in fresh and cryopreserved tissue. Moreover, cryopreservation had little effect on transcriptional profiles, which showed significant overlap between tonsils and LN. The presence and spatial distribution of transcriptionally defined cell types were confirmed by in situ analyses. Our broadly applicable approach promises to greatly enable research into the roles of LNSCs in human disease. © 2023 Wiley-VCH GmbH. |
| Keywords: | adult; child; controlled study; human tissue; preschool child; excision; young adult; human cell; flow cytometry; lymph nodes; cell viability; pathology; transcriptomics; histology; endothelium cell; cryopreservation; lymph node; cell isolation; fibroblast; immunophenotyping; stroma cell; lymphoid tissue; bioinformatics; stromal cells; lymphocyte; lymphocytes; freeze thawing; lymphadenopathy; cell selection; immunofluorescence microscopy; tonsil; cauterization; lymph node cell; high throughput sequencing; ln; humans; human; article; vascular smooth muscle cell; biobank; biological specimen banks; single cell rna seq; paired end sequencing; lymphoid tissue fibroblasts; tonsil disease; tonsillar hyperplasia |
| Journal Title: | European Journal of Immunology |
| Volume: | 53 |
| Issue: | 9 |
| ISSN: | 0014-2980 |
| Publisher: | Wiley V C H Verlag Gmbh |
| Date Published: | 2023-09-01 |
| Start Page: | 2250362 |
| Language: | English |
| DOI: | 10.1002/eji.202250362 |
| PUBMED: | 37366295 |
| PROVIDER: | scopus |
| PMCID: | PMC10529925 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
