Noninvasive in vivo imaging of CD4 cells in simian-human immunodeficiency virus (SHIV)-infected nonhuman primates Journal Article
| Authors: | Di Mascio, M.; Paik, C. H.; Carrasquillo, J. A.; Maeng, J. S.; Jang, B. S.; In, S. S.; Srinivasula, S.; Byrum, R.; Neria, A.; Kopp, W.; Catalfamo, M.; Nishimura, Y.; Reimann, K.; Martin, M.; Lane, H. C. |
| Article Title: | Noninvasive in vivo imaging of CD4 cells in simian-human immunodeficiency virus (SHIV)-infected nonhuman primates |
| Abstract: | Since the earliest days of the HIV epidemic, the number of CD4<sup>+</sup> T cells per unit volume of blood has been recognized as a major prognostic factor for the development of AIDS in persons with HIV infection. It has also been generally accepted that approximately 2% of total body lymphocytes circulate in the blood. In the present study, we have used a nondepleting humanized anti-CD4 monoclonal antibody labeled with the gamma emitter indium-111 to visualize the CD4<sup>+</sup> T-cell pool in vivo in nonhuman primates with simian HIV infection. A strong correlation was noted between radiotracer uptake in spleen, tonsil, axillary lymph nodes, and peripheral blood CD4 T-cell counts (p = 0.75, 0.93, and 0.85, respectively, P < .005). The relationship between radiotracer retention in lymphoid tissues and CD4<sup>+</sup> T-cell counts in the circulation was governed by an exponential law. These data provide an estimate for the total number of lymphocytes in the body as being between 1.9 and 2.9 x 10<sup>12</sup> and suggest that the partition between peripheral blood and lymphoid tissue is between 0.3% and 0.5%. |
| Keywords: | controlled study; nonhuman; human immunodeficiency virus infection; animal cell; animal; animals; animal tissue; cell line; animal experiment; animal model; pathology; virology; monoclonal antibody; axillary lymph node; disease model; immunology; antibodies, monoclonal; immunoglobulin g; drug distribution; drug uptake; isotope labeling; tomography; cd4+ t lymphocyte; cd4-positive t-lymphocytes; substrate specificity; acquired immune deficiency syndrome; cd4 lymphocyte count; cd4 antigen; disease models, animal; rna virus; single photon emission computer tomography; indium 111; okt 4; blood volume; cell suspension; lymphoid tissue; primate; simian human immunodeficiency virus; enzyme specificity; macaca; monkey disease; simian immunodeficiency virus; antigens, cd4; macaca mulatta; simian acquired immunodeficiency syndrome |
| Journal Title: | Blood |
| Volume: | 114 |
| Issue: | 2 |
| ISSN: | 0006-4971 |
| Publisher: | American Society of Hematology |
| Date Published: | 2009-07-09 |
| Start Page: | 328 |
| End Page: | 337 |
| Language: | English |
| DOI: | 10.1182/blood-2008-12-192203 |
| PUBMED: | 19417212 |
| PROVIDER: | scopus |
| PMCID: | PMC2714208 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 3" - "Export Date: 30 November 2010" - "CODEN: BLOOA" - "Source: Scopus" |
