Quantitative collagen analysis using second harmonic generation images for the detection of basal cell carcinoma with ex vivo multiphoton microscopy Journal Article
| Authors: | Sendín-Martín, M.; Posner, J.; Harris, U.; Moronta, M.; Conejo-Mir Sánchez, J.; Mukherjee, S.; Rajadhyaksha, M.; Kose, K.; Jain, M. |
| Article Title: | Quantitative collagen analysis using second harmonic generation images for the detection of basal cell carcinoma with ex vivo multiphoton microscopy |
| Abstract: | Basal cell carcinoma (BCC) is the most common skin cancer, and its incidence is rising. Millions of benign biopsies are performed annually for BCC diagnosis, increasing morbidity, and healthcare costs. Non-invasive in vivo technologies such as multiphoton microscopy (MPM) can aid in diagnosing BCC, reducing the need for biopsies. Furthermore, the second harmonic generation (SHG) signal generated from MPM can classify and prognosticate cancers based on extracellular matrix changes, especially collagen type I. We explored the potential of MPM to differentiate collagen changes associated with different BCC subtypes compared to normal skin structures and benign lesions. Quantitative analysis such as frequency band energy analysis in Fourier domain, CurveAlign and CT-FIRE fibre analysis was performed on SHG images from 52 BCC and 12 benign lesions samples. Our results showed that collagen distribution is more aligned surrounding BCCs nests compared to the skin's normal structures (p < 0.001) and benign lesions (p < 0.001). Also, collagen was orientated more parallelly surrounding indolent BCC subtypes (superficial and nodular) versus those with more aggressive behaviour (infiltrative BCC) (p = 0.021). In conclusion, SHG signal from type I collagen can aid not only in the diagnosis of BCC but could be useful for prognosticating these tumors. Our initial results are limited to a small number of samples, requiring large-scale studies to validate them. These findings represent the groundwork for future in vivo MPM for diagnosis and prognosis of BCC. © 2022 The Authors. Experimental Dermatology published by John Wiley & Sons Ltd. |
| Keywords: | adult; controlled study; human tissue; aged; human cell; major clinical study; cancer diagnosis; protein localization; protein analysis; dermoscopy; basal cell carcinoma; skin neoplasms; pathology; epiluminescence microscopy; collagen type 1; collagen type i; skin tumor; statistical significance; quantitative analysis; cancer infiltration; collagen; cancer classification; ex vivo study; multiphoton microscopy; microscopy, fluorescence, multiphoton; carcinoma, basal cell; diagnostic test accuracy study; collagen fiber; second harmonic generation microscopy; skin structure; procedures; cancer prognosis; benign neoplasm; humans; human; male; female; article; nodular basal cell carcinoma; superficial basal cell carcinoma; second harmonic generation |
| Journal Title: | Experimental Dermatology |
| Volume: | 32 |
| Issue: | 4 |
| ISSN: | 0906-6705 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2023-04-01 |
| Start Page: | 392 |
| End Page: | 402 |
| Language: | English |
| DOI: | 10.1111/exd.14713 |
| PUBMED: | 36409162 |
| PROVIDER: | scopus |
| PMCID: | PMC10478030 |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008748) acknowledged in PubMed and PDF -- MSK corresponding author is Manu Jain -- Export Date: 1 May 2023 -- Source: Scopus |
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