Quantitation of estradiol and testosterone in serum using LC-MS/MS Journal Article

  


Authors: Schofield, R. C.; Kirchoff, D.; Carlow, D. C.
Article Title: Quantitation of estradiol and testosterone in serum using LC-MS/MS
Abstract: Adult and pediatric endocrinology and oncology often requires measuring serum estrogens and testosterone at very low concentrations. Conventional immunoassay methods often lack the required performance to meet this analytical need, and mass spectrometry techniques must be employed. Our aim was to develop a sensitive HPLC-MS/MS assay for both estradiol (E2) and testosterone (Te) in serum, utilizing commercially available calibrators and without the need for chemical derivatization. Serum samples, after the addition of an internal standard, are combined with a hexane:ethyl acetate extraction solution. The samples are vortexed, and the organic layer is decanted into a clean sample tube and evaporated to dryness under a stream of nitrogen. The samples are reconstituted in a water:methanol solution and separated chromatographically using a reversed-phase HPLC column. Subsequent mass spectrometry is performed using both positive ion mode for Te and negative ion mode for E2. © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Keywords: adult; child; mass spectrometry; estrogen; estrogens; standard; reference standards; tandem mass spectrometry; water; chromatography, liquid; estradiol; liquid chromatography; testosterone; serum; nitrogen; methanol; procedures; humans; human; commercial calibrators; hexane; hexanes
Journal Title: Methods in Molecular Biology
Volume: 2546
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2022-01-01
Start Page: 141
End Page: 148
Language: English
DOI: 10.1007/978-1-0716-2565-1_13
PUBMED: 36127585
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85138152028&doi=10.1007%2f978-1-0716-2565-1_13&partnerID=40&md5=a5fd0421fbec7e3358db6e8467dd5871
Notes: Book Chapter 13 in "Clinical Applications of Mass Spectrometry in Biomolecular Analysis - Methods and Protocols. 2nd ed" (ISBN: 978-1-0716-2564-4) -- MSK author Daniel Kirchhoff's last name is misspelled on the original publication -- Export Date: 3 October 2022 -- Source: Scopus
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MSK Authors
  1. Ryan Schofield
    29 Schofield
  2. Dean Christian Carlow
    42 Carlow
  3. Daniel Conrad-Moore Kirchhoff
    3 Kirchhoff
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