Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS Journal Article
| Authors: | Schofield, R. C.; Ramanathan, L. V.; Pessin, M. S.; Carlow, D. C. |
| Article Title: | Data to support a simultaneous testosterone and estradiol assay in serum by LC-MS/MS |
| Abstract: | A very sensitive LC-MS/MS assay was developed implementing a liquid-liquid extraction step followed by mass spectrometry which was operated in both positive and negative ion modes. The assay was calibrated with readily available commercial calibrators and compared with international reference standards. This data is also presented in “Sensitive Simultaneous Quantitation of Testosterone and Estradiol in Serum by LC-MS/MS without Derivatization and Comparison with the CDC HoSt Program” (Schofield et al., 2017). This article includes the comparison of the LC-MS/MS assay with a commonly available chemiluminescencent immunoassay for the quantitation of both estradiol and testosterone. In addition we show baseline separation of estradiol and testosterone from other structurally related and/or isobaric compounds that could potentially interfere with the assay. In addition, various calibrator materials were tested and compared with internationally-recognized reference materials. © 2018 The Authors |
| Keywords: | mass spectrometry; estradiol; liquid chromatography; testosterone; method correlation; nist srm 971; bcr 571–579 |
| Journal Title: | Data in Brief |
| Volume: | 20 |
| ISSN: | 2352-3409 |
| Publisher: | Elsevier B.V. |
| Date Published: | 2018-10-01 |
| Start Page: | 1160 |
| End Page: | 1165 |
| Language: | English |
| DOI: | 10.1016/j.dib.2018.08.077 |
| PROVIDER: | scopus |
| PMCID: | PMC6141700 |
| PUBMED: | 30246107 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 October 2018 -- Source: Scopus |
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42Carlow
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