Quantitation of 25-OH-vitamin-D(2) and 25-OH-Vitamin-D(3) in urine using LC-MS/MS Journal Article
| Authors: | Carlow, D. C.; Schofield, R. C.; Denburg, M. |
| Article Title: | Quantitation of 25-OH-vitamin-D(2) and 25-OH-Vitamin-D(3) in urine using LC-MS/MS |
| Title Series: | Methods in Molecular Biology |
| Abstract: | Patients with significant proteinuria represent a unique population with respect to vitamin D status due to the urinary losses of vitamin D-binding protein (DBP) to which >99 % of circulating 25-hydroxy vitamin D (25(OH)D) is bound. Low serum concentrations of 25(OH) D have been found in children and adults with nephrotic syndrome (NS). However, previously described assays developed to quantify the magnitude of urinary loss are technically challenging. This chapter describes a simple and sensitive method to quantify 25(OH)D-2 and 25(OH)D-3 in urine specimens in a single analytical LC-MS/MS analysis. This assay is more sensitive than previously described radioimmunoassays and offers the ability to quantitate both forms of 25-hydroxy vitamin D. The assay involves no chemical derivitization, has a linear measurement range of 20-1500 pg/mL and displays imprecision (CVs) below 7 % at various concentrations across the analytical measurement range. |
| Keywords: | mass spectrometry; urine; liquid chromatography; nephrotic syndrome; disease; 25-hydroxy vitamin d-2; 25-hydroxy vitamin d-3; vitamin-d metabolites; normal renal-function; d-binding protein; calcium-metabolism; bone-histology |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1378 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Publication Place: | New York, NY |
| Date Published: | 2016-01-01 |
| Start Page: | 321 |
| End Page: | 329 |
| Language: | English |
| ACCESSION: | WOS:000368838500034 |
| PROVIDER: | wos |
| PUBMED: | 26602143 |
| DOI: | 10.1007/978-1-4939-3182-8_33 |
| Notes: | Article; Book Chapter 33 in "Clinical Applications of Mass Spectrometry in Biomolecular Analysis" (ISBN: 978-1-4939-3181-1) -- 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA -- Source: Wos |
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