Simultaneous determination of meso-tetraphenylporphyrine and Co(II)-meso-tetraphenylporphyrine by high performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry Journal Article

  


Authors: Xiao, J. B.; Jiang, X. Y.; Chen, X. Q.; Chen, P.; Xu, M.
Article Title: Simultaneous determination of meso-tetraphenylporphyrine and Co(II)-meso-tetraphenylporphyrine by high performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry
Abstract: A high performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) method without sample pretreatment was developed and validated for the determination of 5,10,15,20-mesotetraphenylporphyrine (TPP) and Co(II)- 5,10,15,20-meso- tetraphenylporphyrine (CoTPP) in the catalyst. Samples were dissolved in CH 2Cl2 and were directly injected into the HPLC-DAD-ESI-MS system in which an isocratic elution program was applied. The mobile phase was methanol-acetonitrile-chloroform (70:30:10, v/v). The mass spectrometer was operated in ESI monitoring mode. TPP and CoTPP were detected with excellent sensitivity and selectivity. The use of UV-Vis diode array allowed the detection of the analytes within the 0.5-15.4 μg/mL range for TPP and 0.8-20.6 μg/mL range for CoTPP. The lower limits of quantification were 0.076 μg/ml for TPP and 0.32 μg/ml for CoTPP. The results pointed out that the method was suitable for the catalyst and allowed their identification and quantification with no need for purification steps.
Keywords: co(ii)-mesotetraphenyl porphyrine; hplc-dad-esi-ms; meso-tetraphenylporphyrine
Journal Title: Canadian Journal of Analytical Sciences and Spectroscopy
Volume: 51
Issue: 3
ISSN: 1205-6685
Publisher: Canadian Soc Analytical Sciences & Spectroscopy  
Date Published: 2006-01-01
Start Page: 164
End Page: 173
Language: English
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-72149101501&partnerID=40&md5=75c937925e2f5e7b2754951d9fa35c5d
Notes: --- - "Export Date: 4 June 2012" - "Source: Scopus"