High-throughput, parallel flow cytometry screening of hundreds of cell surface antigens using fluorescent barcoding Journal Article
| Authors: | Drápela, S.; Fedr, R.; Vacek, O.; Remšík, J.; Souček, K. |
| Article Title: | High-throughput, parallel flow cytometry screening of hundreds of cell surface antigens using fluorescent barcoding |
| Abstract: | Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | flow cytometry; biomarkers; biological marker; fluorescent dye; fluorescent dyes; membrane antigen; research; antigens, surface; high-throughput screening; multicolor flow cytometry; cell surface phenotyping; fluorescent cell barcoding |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 2543 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2022-01-01 |
| Start Page: | 99 |
| End Page: | 111 |
| Language: | English |
| DOI: | 10.1007/978-1-0716-2553-8_9 |
| PUBMED: | 36087262 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Book Chapter 9 in "Apoptosis and Cancer" (ISBN: 978-1-0716-2552-1) -- Export Date: 3 October 2022 -- Source: Scopus |
