Imaging flow cytometry to assess antigen-presenting-cell function Review


Authors: Markey, K. A.; Gartlan, K. H.
Review Title: Imaging flow cytometry to assess antigen-presenting-cell function
Abstract: This unit describes methods for quantifying phagocytosis and imaging the immunological synapse between T cells and antigen-presenting cells (APCs), with both techniques delivering valuable information about APC function. These aspects of APC biology have traditionally been challenging to quantify, and imaging flow cytometry, which harnesses the high-throughput nature of flow cytometry combined with the capacity of microscopy to deliver spatial localization, facilitates analysis of these APC functions in a fashion that was previously not possible. Imaging flow cytometry allows large numbers of events to be captured and large amounts of fluorescence data to be quantified at the physical location of markers of interest, both on the cell surface and in intracellular compartments, combining key features of traditional flow cytometry and fluorescence microscopy. © 2019 by John Wiley & Sons, Inc. © 2019 John Wiley & Sons, Inc.
Keywords: human cell; flow cytometry; t lymphocyte; cell function; quantitative analysis; fluorescence microscopy; phagocytosis; antigen presenting cell; cell surface; immunological synapse; article; imagestream; imaging flow cytometry
Journal Title: Current Protocols in Immunology
Volume: 125
Issue: 1
ISSN: 1934-3671
Publisher: John Wiley & Sons  
Date Published: 2019-06-01
Start Page: e72
Language: English
DOI: 10.1002/cpim.72
PROVIDER: scopus
PUBMED: 30840360
DOI/URL:
Notes: Source: Scopus
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  1. Kate Ann Markey
    39 Markey