Comparative analysis of surface membrane immunoglobulin determination by flow cytometry and fluorescence microscopy Journal Article

  


Authors: Gebhard, D. F. Jr; Mittelman, A.; Cirrincione, C.; Thaler, H. T.; Koziner, B.
Article Title: Comparative analysis of surface membrane immunoglobulin determination by flow cytometry and fluorescence microscopy
Abstract: The analysis of membrane surface immunoglobulin (SmIg) on B lymphocytes was carried out in 59 normal individuals and nine patients with B-cell non-Hodgkin's lymphomas by conventional immunofluorescence microscopy and flow cytometry. Five channel settings of a cytofluorograph were evaluated (100, 150, 200, 250, 300) and the mean and standard deviation of the percent positive cells were calculated and compared to the mean and standard deviation of th microscope reading. On the basis of the relative fluorescence reactivity, we were able to determine a fluorescence intensity at which the results of flow cytometry and fluorescence microscopy were comparable. In normal individuals, for cells expressing surface Ia, the channel giving similar results to that of fluorescence microscopy was 150; for kappa and lambda chains, channel 200; for Fab'PV, channel 200; and for IgM, channel 250. In patients with B-cell non-Hodgkin's lymphomas, for cells expressing surface Ia the channel giving similar results to that of fluorescence microscopy was 100; for kappa, channel 100; for lambda, channel 200; for Fab'FV, channel 150; and for IgM, channel 150. Flow cytometric analysis of SmIg appears to be superior to fluorescence microscopy in efficiency, and has the added advantages of being a rapid, sensitive, and objectively quantitative methodology.
Keywords: clinical article; human cell; flow cytometry; immunoglobulin; b lymphocyte; lymphatic system; nonhodgkin lymphoma; diagnosis; fluorescence microscopy; cell surface; human; priority journal; blood and hemopoietic system
Journal Title: Journal of Histochemistry and Cytochemistry
Volume: 34
Issue: 4
ISSN: 0022-1554
Publisher: Sage Publications  
Date Published: 1986-04-01
Start Page: 475
End Page: 481
Language: English
DOI: 10.1177/34.4.3081624
PROVIDER: scopus
PUBMED: 3081624
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022650098&doi=10.1177%2f34.4.3081624&partnerID=40&md5=57988090f0fc860895398398e4f1cf6b
Notes: Article -- Export Date: 18 August 2021 -- Source: Scopus
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MSK Authors
  1. Howard T Thaler
    245 Thaler
  2. Benjamin Koziner
    52 Koziner
  3. Constance Cirrincione
    21 Cirrincione
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