Synthesis and morphological studies of Tc-99m-labeled lupulone-conjugated Fe(3)O(4)@TiO(2) nanocomposite, and in vitro cytotoxicity activity on prostate cancer cell lines Journal Article


Authors: Tutun, E.; Tekin, V.; Yasakcı, V.; Aras, Ö; Ünak, P.
Article Title: Synthesis and morphological studies of Tc-99m-labeled lupulone-conjugated Fe(3)O(4)@TiO(2) nanocomposite, and in vitro cytotoxicity activity on prostate cancer cell lines
Abstract: The purpose of this study was to develop a multifunctional theranostic probe for imaging (magnetic resonance imaging [MRI] and single-photon emission computed tomography [SPECT]) and therapy (photodynamic therapy). For this purpose, Tc-99m-labeled lupulone-conjugated Fe3O4@TiO2 nanocomposites (99mTc-DTPA-Fe3O4@TiO2-HLP and 99mTc-DTPA-Fe3O4@TiO2-ALP nanocomposites) were synthesized. The average diameter of the nanocomposites was 171 ± 20 nm as seen on transmission electron microscopy images. Fe3O4@TiO2 nanocomposites exhibited fluorescence spectra at an emission wavelength of 314 nm. Lupulone-conjugated Fe3O4@TiO2 nanocomposites were spherical-shaped with a suitable dispersion and without visible aggregation, and their radiolabeling yields were over 85%. Healthy (RWPE-1 normal human prostate epithelial cell line) and cancer prostate cell lines (PC-3 human prostate cancer cell line) were used to determine the in vitro biological behavior of the nanocomposites. The PC-3 cells treated with lupulone-conjugated Fe3O4@TiO2 nanocomposites showed a lower cell viability compared with RWPE-1 cells treated with lupulone-conjugated Fe3O4@TiO2 nanocomposites. Lupulone-modified Fe3O4@TiO2 nanocomposites may serve in the future as a multifunctional probe for positron emission tomography (PET)/MRI, photodynamic therapy, and hyperthermia therapy of cancer. © 2021 John Wiley & Sons, Ltd.
Keywords: positron emission tomography; magnetic resonance imaging; cell culture; urology; probes; photodynamic therapy; diseases; positron emission tomography (pet); theranostics; iron oxides; emission wavelength; cells; titanium dioxide; single photon emission computed tomography; hyperthermia therapy; biological behavior; tc-99m; human prostate cancer cells; nanocomposites; prostate cancer cells; fluorescence spectra; tio2 nanoparticles; particle beams; high resolution transmission electron microscopy; fe3o4@tio2; magnetite; lupulone; oxide minerals; multifunctional probes; transmission electron microscopy images
Journal Title: Applied Organometallic Chemistry
Volume: 35
Issue: 12
ISSN: 0268-2605
Publisher: John Wiley & Sons  
Date Published: 2021-12-01
Start Page: e6435
Language: English
DOI: 10.1002/aoc.6435
PROVIDER: scopus
PMCID: PMC9797211
PUBMED: 36582207
DOI/URL:
Notes: Article -- Export Date: 3 January 2022 -- Source: Scopus
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  1. Omer Aras
    75 Aras