Specificity analysis of mouse monoclonal antibodies defining cell surface antigens of human renal cancer Journal Article


Authors: Finstad, C. L.; Cordon-Cardo, C.; Bander, N. H.; Whitmore, W. F.; Melamed, M. R.; Old, L. J.
Article Title: Specificity analysis of mouse monoclonal antibodies defining cell surface antigens of human renal cancer
Abstract: Six mouse monoclonal antibodies (mAbs) defining separate systems of cell surface antigens of cultured human renal cancer were tested for reactivity with normal fetal and adult tissues and with neoplastic tissues. Five of the mAbs identified glycoproteins of M(r) 160,000 (designated S4), M(r) 140,000 (F23), M(r) 120,000 (S23 and S27), and M(r) 115,000 (S22). The glycoprotein component of M(r) 120,000 has been shown recently to be the adenosine deaminase binding protein (ADA-BP) and mAbS23 and mAbS27 define two distinct epitopes on ADA-BP. S22 was not detected on any normal fetal or adult tissues but was found on a subset of renal cancers. S4, F23, S23, and S27 defined distinct domains of the nephron: glomerulus (S4), proximal tubules (S4, F23, S23, and S27), and portions of Henle's loop (S23 and S27). mAbS4 also reacted with the interstitial matrix in the renal medulla and of other tissues, and mAbF23 reacted with fetal and adult fibroblasts. The S23 epitope of ADA-BP was expressed by placental trophoblasts and epithelial cells of breast, prostate, lung, and colon, whereas the S27 epitope was detected on a more limited range of cell types (trophoblasts and prostate epithelium). A panel of 20 renal cell carcinomas was typed for expression of these antigens; 7 phenotypes could be distinguished, with the S4+/F23+/S23+/S27+/S22+ or - phenotype (15 cases) being most common. The other antigenic system, V1, identified a heat-stable antigen that was widely expressed on cultured cell types but showed a restricted pattern of reactivity in tissues. V1 expression was limited to the adrenal cortex, Leydig cells, and the theca of ovarian follicles, and to adrenal cortical carcinomas.
Keywords: human cell; mouse; animals; mice; neoplasm proteins; cell line; membrane proteins; kidney neoplasms; monoclonal antibody; membrane antigen; antibodies, monoclonal; kidney; antigens, neoplasm; organ specificity; antibody specificity; kidney cancer; molecular weight; glycoproteins; antigens, surface; adenosine deaminase; glomerulus; humans; human; male; female; priority journal; ada
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 82
Issue: 9
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1985-05-01
Start Page: 2955
End Page: 2959
Language: English
DOI: 10.1073/pnas.82.9.2955
PROVIDER: scopus
PMCID: PMC397685
PUBMED: 3857626
DOI/URL:
Notes: Article -- Export Date: 26 October 2021 -- Source: Scopus
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MSK Authors
  1. Neil Harrison Bander
    62 Bander
  2. Lloyd J Old
    593 Old
  3. Willet F. Whitmore Jr
    139 Whitmore
  4. Connie L. Finstad
    45 Finstad
  5. Myron Melamed
    148 Melamed