Initial immunochemical characterization of MX35 ovarian cancer antigen Journal Article
| Authors: | Welshinger, M.; Yin, B. W. T.; Lloyd, K. O. |
| Article Title: | Initial immunochemical characterization of MX35 ovarian cancer antigen |
| Abstract: | Monoclonal antibody (mAb) MX35 reacts with approximately 90% of ovarian epithelial cancers and has been studied in localization and biodistribution trials in ovarian cancer patients. This study shows that mAb MX35 recognizes a cell surface antigen of about 95,000 D on OVCAR-3 ovarian cancer cells. The antigen could be immunoprecipitated from lysates of cells metabolically labeled with [3H]glucosamine and it bound to concanavalin A and wheat germ agglutinin lectins, showing that it is a glycoprotein. MX35 antigen can also be detected in detergent lysates of OVCAR-3 cells by Western blotting. Using this technique the MX35 epitope(s) was shown to be heat stable but susceptible to reduction by 2-mercaptoethanol. Protease digestion of the antigen resulted in smaller fragments (42-52 kDa) that still reacted with antibody. We conclude that MX35 antigen is a 95 kDa glycoprotein, stabilized by disulfide bonds, with a large protease-resistant region that carries the MX35 epitopes. |
| Keywords: | human cell; antigen expression; ovarian neoplasms; ovary cancer; tumor cells, cultured; tumor antigen; monoclonal antibody; antigens, neoplasm; immunoprecipitation; epithelium cell; receptor affinity; molecular weight; immunochemistry; cell lysate; cell surface; epitopes; disulfide bond; precipitin tests; humans; human; female; priority journal; article |
| Journal Title: | Gynecologic Oncology |
| Volume: | 67 |
| Issue: | 2 |
| ISSN: | 0090-8258 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1997-11-01 |
| Start Page: | 188 |
| End Page: | 192 |
| Language: | English |
| DOI: | 10.1006/gyno.1997.4846 |
| PUBMED: | 9367706 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 17 March 2017 -- Source: Scopus |
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