DNA-mediated gene transfer of a human cell surface 170-kilodalton glycoprotein: Evidence for association with an endogenous murine protein Journal Article
| Authors: | Kantor, R. R. S.; Bander, N. H.; Finstad, C. L.; Graf, L. H. Jr; Lloyd, K. O.; Old, L. J.; Albino, A. P. |
| Article Title: | DNA-mediated gene transfer of a human cell surface 170-kilodalton glycoprotein: Evidence for association with an endogenous murine protein |
| Abstract: | We have previously reported the identification and characterization of two related human cell surface protein complexes, very common antigens 1 and 2 (VCA-1, VCA-2) (Kantor, R.R.S., Mattes, M.J., Lloyd, K.O., Old L.J., and Albino, A.P. (1987) J. Biol. Chem. 262, 15158-15165). We now report the transfection of DNA sequences encoding to 170-kilodalton heterodimer of VCA-2 from human SK-RC-41 renal cancer cells to B78H1 mouse melanoma cells. B78H1 cells were cotransfected with high molecular weight renal cancer DNA and a plasmid vector containing the neomycin resistance gene. Antibiotic-resistant transfectants were screened for the expression of the 170-kDa heterodimer with mouse monoclonal antibody (mAb) J143. Analysis of mAb J143-positive (J143+) transfectants showed that they expressed a 170-kDa heterodimer with an identical molecular weight, isoelectric point, two-dimensional peptide map, and spatial orientation of surface-exposed epitopes to the homologous 170-kDa species seen in human donor cells. The 170-kDa heterodimer in SK-RC-41 cells is associated with a 140-kDa (designated 1401) polypeptide to form the VCA-2 complex. The 170-kDa complex and the 1401-kDa polypeptides are encoded by genes located on different human chromosomes. J143+ transfectants display a molecule of 140 kDa associated with the 170-kDa complex which is biochemically similar but non-identical, to the human 1401-kDa polypeptide on VCA-2. This evidence supports our interpretation that the transfected human 170-kDa heterodimer associates with a murine counterpart of the human 1401-kDa polypeptide in J143+ transfectants. |
| Keywords: | animal; mice; heredity; in vitro study; transfection; gene transfer; fluorescent antibody technique; antibodies, monoclonal; dna; membrane glycoproteins; molecular weight; antigens, surface; cell surface protein; human; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 262 |
| Issue: | 31 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1987-11-05 |
| Start Page: | 15166 |
| End Page: | 15171 |
| Language: | English |
| PUBMED: | 3312201 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S0021-9258(18)48153-7 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work