Biochemical analysis of two cell surface glycoprotein complexes, very common antigen 1 and very common antigen 2: Relationship to very late activation T cell antigens Journal Article
| Authors: | Kantor, R. R. S.; Mattes, M. J.; Lloyd, K. O.; Old, L. J.; Albino, A. P. |
| Article Title: | Biochemical analysis of two cell surface glycoprotein complexes, very common antigen 1 and very common antigen 2: Relationship to very late activation T cell antigens |
| Abstract: | Two mouse monoclonal antibodies (mAb), AJ2 and J143, define two related human cell surface protein complexes, very common antigen 1 (VCA-1) and very common antigen 2 (VCA-2). In the present report, these complexes have been defined with respect to: (i) subunit arrangement; (ii) monoclonal antibody binding sites; (iii) carbohydrate content; (iv) homology to other cell surface protein complexes; and (v) possible functional roles. Analysis of the antigens from a human melanoma cell line, MeWo, reveals that VCA-1 is a noncovalently linked heterodimer of 170- and 140 (designated 1401)-kDa polypeptides. mAb AJ2 reacts with an epitope on the 1401-kDa polypeptide. VCA-2 is composed of the same 1401-kDa polypeptide as VCA-1 and another 170-kDa species; this 170-kDa species consists of a second distinct 140-kDa (designated 1402) and a 30-kDa polypeptide which are disulfide-bonded. Indirect evidence indicates that mAb J143 reacts with an epitope on this 170-kDa complex. Peptide mapping has shown that the complexes are members of a family of cell surface proteins that include antigens present on activated T cells (designated very late activation antigens). Since VCA-2 is found predominantly on the basal membrane of adherent cells and its expression increases 12-fold when HUT-102 lymphoblastoid cells are grown as an adherent cell culture, we suggest that VCA-2 plays a role in cellular adherence. |
| Keywords: | t-lymphocytes; cell line; membrane antigen; antibodies, monoclonal; membrane glycoproteins; cell membrane; cell adhesion; molecular weight; glycoprotein; carbohydrates; antigens, surface; t lymphocyte antigen; human; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; macromolecular systems; blood and hemopoietic system |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 262 |
| Issue: | 31 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1987-11-05 |
| Start Page: | 15158 |
| End Page: | 15165 |
| Language: | English |
| PUBMED: | 3499438 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S0021-9258(18)48152-5 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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