Cloning and characterization of a cDNA encoding the rat brain glucose-transporter protein Journal Article
| Authors: | Birnbaum, M. J.; Haspel, H. C.; Rosen, O. M. |
| Article Title: | Cloning and characterization of a cDNA encoding the rat brain glucose-transporter protein |
| Abstract: | Antibody raised against the human erythrocyte glucose transporter identified a recombinant λgt11 bacteriophage in a cDNA library prepared from immunoselected polysomal RNA from adult rat brain. The cDNA predicts a 492-amino acid protein that demonstrates 97.6% identity to the human hepatoma hexose carrier. The tissue distribution of the transporter mRNA is identical to that of immunologically identifiable protein and transport activity, except in liver in which high levels of transport are associated with little or no transporter mRNA or protein. As assayed by blot-hybridization analysis, mRNA from insulin-responsive and nonresponsive tissues are indistinguishable. These data suggest that a genetically unrelated protein is responsible for hexose transport in normal liver. |
| Keywords: | nonhuman; animal cell; cytology; heredity; central nervous system; animalia; molecular cloning; liver; genetic engineering; brain; protein synthesis; nucleotide sequence; rat; radioisotope; complementary dna; gene structure; normal value; recombinant dna; priority journal; hexose |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 83 |
| Issue: | 16 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1986-08-01 |
| Start Page: | 5784 |
| End Page: | 5788 |
| Language: | English |
| DOI: | 10.1073/pnas.83.16.5784 |
| PUBMED: | 3016720 |
| PROVIDER: | scopus |
| PMCID: | PMC386379 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus |
