Independent regulation of B-cell inducing factor and IL-2 production by T lymphocytes, and direct and indirect promotion of immunoglobulin secretion by glucocorticosteroid Journal Article
| Authors: | Jeong, G.; Nakoinz, I.; Ralph, P. |
| Article Title: | Independent regulation of B-cell inducing factor and IL-2 production by T lymphocytes, and direct and indirect promotion of immunoglobulin secretion by glucocorticosteroid |
| Abstract: | The conditions for induction of B-cell inducing factor (BIF) by human peripheral blood T cells was investigated. BIF was assayed by induction of immunoglobulin secreting cells (ISC) in peripheral blood B (non-T) cells stimulated with Staphylococcus aureus bacteria strain Cowan I (Sac), and in the IgM cell line SKW6.4. Maximum BIF production occurred with high concentrations of the T-cell mitogens phytohemagglutinin, concanavalin A, and PWM. Dexamethasone (Dex) also induced BIF production in T cells at 10-5 to 10-7 M. At 10-5 and 10-6 M Dex, the T-cell supernatants had to be dialyzed before testing because Dex alone stimulated variable levels of ISC in both test B-cell assays. Dex did not enhance BIF production by T cells that were optimally stimulated by lectin. BIF levels were maximum by Day 2 of T-cell cultures and remained high at Days 3 and 4. In contrast, IL-2 reached a peak at Day 1 and declined drastically by Day 4. We previously showed that IL-2 at less than 100 U/ml did not induce ISC in B cells and did not alter ISC induction by BIF. Dex did not induce IL-2 production and inhibited IL-2 production induced by Con A, in contrast to the promoting effects of Dex on BIF production, providing further evidence for the independence of BIF and IL-2 production and B-cell stimulation. © 1986. |
| Keywords: | adult; human cell; drug efficacy; t lymphocyte; t-lymphocytes; cells, cultured; interleukin 2; dexamethasone; in vitro study; b lymphocyte; b-lymphocytes; lymphatic system; glucocorticoid; drug monitoring; drug sensitivity; therapy; interleukin-2; interleukin-4; growth substances; phytohemagglutinin; topical drug administration; lectins; concanavalin a; human; priority journal; lymphokines; immunoglobulin production; lymphocyte cooperation; preliminary communication; pokeweed mitogen |
| Journal Title: | Cellular Immunology |
| Volume: | 103 |
| Issue: | 1 |
| ISSN: | 0008-8749 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1986-11-01 |
| Start Page: | 199 |
| End Page: | 206 |
| Language: | English |
| DOI: | 10.1016/0008-8749(86)90081-x |
| PUBMED: | 3492284 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 18 August 2021 -- Source: Scopus; Acknowledgments: We thank Ruth Bengelsdorf for manuscript preparation and editing and the Cetus Assay Lab for IL-2 determination. |
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