Cytokine production by CD4+ T-cells responding to antigen presentation by melanoma cells Journal Article
| Authors: | Brady, M. S.; Eckels, D. D.; Lee, F.; Ree, S. Y.; Lee, J. S. |
| Article Title: | Cytokine production by CD4+ T-cells responding to antigen presentation by melanoma cells |
| Abstract: | Melanoma cells are unusual because, unlike most epithelial tumours, constitutive expression of HLA class II antigens is common. We have previously demonstrated that a peptide-specific CD4+ T-cell clone proliferates briskly in response to peptide and HLA class II expressing melanoma cell lines derived from metastases. Here we demonstrate that these CD4+ T-cells secrete large amounts of interferon-γ (IFNγ) and interleukin- 10 (IL10), and insignificant quantities of IL2 or IL4, in response to peptide presentation by both melanoma and autologous B-cells. T-cells produced more IL10 when responding to peptide presentation by melanoma cells compared with B-cells, and less IFNγ (P<0.01). Addition of IL12 did not alter the cytokines produced but increased the T-cell production of both, especially the production of IL10 in response to peptide presentation by melanoma cells. Our data suggest that differential cytokine production by CD4+ T-cells in response to peptide presentation by HLA class II expressing tumour cells may contribute to tolerance to tumour antigens. |
| Keywords: | human cell; interferon; cell proliferation; t lymphocyte; melanoma; reverse transcription polymerase chain reaction; interleukin 10; cell line; tumor cells, cultured; tumor antigen; cytokine; cytokines; gamma interferon; reverse transcriptase polymerase chain reaction; hla antigen class 2; cd4-positive t-lymphocytes; cytokine production; antigen presenting cell; antigen-presenting cells; lymphocyte count; interleukin-2; interleukin-10; hla system; interleukin-4; cell cloning; interleukin-12; interferon type ii; humans; human; priority journal; article; cd4+ t-cells; hla class ilinterleukin-10 |
| Journal Title: | Melanoma Research |
| Volume: | 9 |
| Issue: | 2 |
| ISSN: | 0960-8931 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1999-01-01 |
| Start Page: | 173 |
| End Page: | 180 |
| Language: | English |
| PUBMED: | 10380940 |
| PROVIDER: | scopus |
| DOI: | 10.1097/00008390-199904000-00010 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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