MHC class II-mediated antigen presentation by melanoma cells Journal Article
| Authors: | Brady, M. S.; Eckels, D. D.; Ree, S. Y.; Schultheiss, K. E.; Lee, J. S. |
| Article Title: | MHC class II-mediated antigen presentation by melanoma cells |
| Abstract: | Constitutive expression of major histocompatibility complex (MHC) class II molecules is normally restricted to professional antigen-presenting cells (APCs) of the immune system, although it also occurs frequently in melanoma. Clinical evidence suggesting that MHC class II expression by melanoma is associated with tumor progression led us to postulate a role for MHC class II-mediated antigen presentation in this disease. First, we investigated whether melanoma cells derived from metastases can process antigen and/or present peptide vi MHC class II molecules to a peptide-specific CD4+ T-cell clone. In all cell lines tested, melanoma cells were able to process antigen and present peptide efficiently to CD4+ T cells, resulting in T-cell proliferation increased 5-26-fold over controls. Next, we found that CD28- mediated costimulation was not required, because blocking with CTLA-4Ig had no effect on the T-cell response to either melanoma or B cells as APCs. In contrast, blocking CD54 (ICAM-1) resulted in a decrease in proliferation in response to peptide presentation by melanoma but not B cells. These data demonstrate that MHC class II molecules on melanoma cells are functional and that antigen-processing pathways are intact. In addition, CD54 seems to play a significant role in peptide presentation by melanoma. |
| Keywords: | controlled study; nonhuman; antigen expression; animal cell; melanoma; skin neoplasms; tumor cells, cultured; b-lymphocytes; antigen presentation; lymphocyte activation; immune tolerance; amino acid sequence; molecular sequence data; major histocompatibility antigen class 2; cd4-positive t-lymphocytes; peptides; histocompatibility antigens class ii; major histocompatibility complex; intercellular adhesion molecule 1; antigen-presenting cells; antigens, cd28; b7 antigen; lymphocyte function-associated antigen-1; cancer immunology; cd80; humans; human; priority journal; article; cd86; mhc class ii; cd54; ctla- 4ig |
| Journal Title: | Journal of Immunotherapy |
| Volume: | 19 |
| Issue: | 6 |
| ISSN: | 1524-9557 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 1996-11-01 |
| Start Page: | 387 |
| End Page: | 397 |
| Language: | English |
| DOI: | 10.1097/00002371-199611000-00001 |
| PUBMED: | 9041456 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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