Proliferation of leukemic B cells in response to SAC and anti-μ. Evidence for different modes of action and comparison to proliferation and differentiation induced by conditioned medium Journal Article
| Authors: | Steinberg, J.; Bona, C.; Polyzos, A.; Moore, M. A. S.; Platsoucas, C. D. |
| Article Title: | Proliferation of leukemic B cells in response to SAC and anti-μ. Evidence for different modes of action and comparison to proliferation and differentiation induced by conditioned medium |
| Abstract: | We investigated the proliferative responses and immunoglobulin production of highly purified E-rosette negative largely leukemic B cells from patients with CLL to Staphylococcus aureus Cowan I (SAC) or to SAC in combination with anti-μ or conditioned medium (CM). The latter was derived by stimulating human peripheral blood mononuclear leukocytes with PHA. We observed: (1) that purified E-rosette negative largely leukemic B cells from 25% (five out of 20) of the patients exhibited proliferative responses to SAC; (2) inhibition of SAC-induced proliferation by anti-μ in certain patients, whereas synergism between SAC and anti-μ in inducing proliferative responses in others; (3) the lack of synergism between SAC and CM in inducing proliferative responses, which is in contrast to the strong synergism that was observed between anti-μ and CM in inducing proliferation; and (4) induction or enhancement by SAC alone of Ig production by largely leukemic B-cell populations from few patients with CLL and purified tonsillar B lymphocytes, but not peripheral blood B cells from normal donors. These results suggest that SAC and anti-μ induce proliferation of B cells by different mechanisms and that B-cell proliferation and differentiation is dependent not only on the mitogen but also on the activation state of the cells. © 1988. |
| Keywords: | human cell; comparative study; cytology; cell growth; cell differentiation; tumor cells, cultured; b lymphocyte; b-lymphocytes; drug synergism; lymphocyte activation; cell culture; leukemia cell; rosette formation; staphylococcus aureus; adjuvants, immunologic; chronic lymphatic leukemia; growth factor; culture media; immunoglobulins; immunoglobulin m; chronic lymphocytic leukemia; growth inhibitors; antibodies, anti-idiotypic; growth factors; human; leukemia, b-cell, chronic; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; immunoglobulin production; conditioned medium; leukemic b-cell proliferation and differentiation; staphylococcus aureus cowan |
| Journal Title: | Leukemia Research |
| Volume: | 12 |
| Issue: | 7 |
| ISSN: | 0145-2126 |
| Publisher: | Elsevier Ltd |
| Date Published: | 1988-01-01 |
| Start Page: | 559 |
| End Page: | 566 |
| Language: | English |
| DOI: | 10.1016/0145-2126(88)90085-9 |
| PUBMED: | 3262791 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
