Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of methicillin-resistant Staphylococcus aureus by resonance light scattering Journal Article
| Authors: | Xiao, J. B.; Chen, J. W.; Ren, F. L.; Yang, C. S.; Xu, M. |
| Article Title: | Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of methicillin-resistant Staphylococcus aureus by resonance light scattering |
| Abstract: | A rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) using resonance light scattering (RLS) on an ordinary fluorescence spectrometer was developed. The viable MRSA reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to produce insoluble particles which displayed intense resonance scattering light. It showed a linear relationship between the number of viable MRSA and the RLS intensity. Dead MRSA were unable to reduce MTT. MRSA exposed to flavonoids extracted from Marchantia convoluta (MCF) showed a MCF concentration-dependent inhibition of the ability to reduce MTT. The RLS could, in combination with the MTT assay, be a rapid and sensitive detection method for vitro-cultured MRSA. © 2007 Elsevier B.V. All rights reserved. |
| Keywords: | nonhuman; sensitivity and specificity; accuracy; cell viability; fluorescence; cell culture; bacterium detection; flavonoid; flavonoids; detection; methicillin resistance; staphylococcus aureus; methicillin resistant staphylococcus aureus; methicillin-resistant staphylococcus aureus; thiazoles; concentration response; resonance; 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; solubility; reduction; bacterial metabolism; tetrazolium salts; scattering, radiation; bacteria; light; light scattering; marchantia convoluta; convoluta; marchantia; resonance light scattering; colony count, microbial; spectrophotometry, ultraviolet; insoluble particles; marchantia convoluta (mcf); methicillin resistant staphylococcus aureus (mrsa); resonance light scattering (rls) |
| Journal Title: | Analytica Chimica Acta |
| Volume: | 589 |
| Issue: | 2 |
| ISSN: | 0003-2670 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2007-04-25 |
| Start Page: | 186 |
| End Page: | 191 |
| Language: | English |
| DOI: | 10.1016/j.aca.2007.02.073 |
| PUBMED: | 17418180 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 11" - "Export Date: 17 November 2011" - "CODEN: ACACA" - "Source: Scopus" |
