A new method for antimicrobial susceptibility testing of vitro-cultured bacteria by means of resonance light scattering technique Journal Article
| Authors: | Shi, Y. J.; Chen, J.; Xu, M. |
| Article Title: | A new method for antimicrobial susceptibility testing of vitro-cultured bacteria by means of resonance light scattering technique |
| Abstract: | A new method for antimicrobial susceptibility testing of vitro-cultured bacteria on an ordinary fluorescence spectrometer was developed. The viable bacteria reduced 3-(4,5-dimethylthiazol -2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to produce insoluble particles that displayed intense resonance scattering light. The assay showed a linear relationship between the number of viable bacteria and the intensity of resonance scattering light. Dead bacteria were unable to reduce MTT. Methicillin-resistant Staphylococcus aureus exposed to flavonoids from Marchantia convoluta showed a flavonoids concentration-dependent inhibition of the ability to reduce MTT. In the assay, less than 12 h was required to attain susceptibility results and fewer bacteria were utilized than in traditional methods. The RLS technique could, in combination with the MTT assay, be a rapid and sensitive measuring method to determine the in vitro activity of new antimicrobials. © The Korean Society for Microbiology and Biotechnology. |
| Keywords: | controlled study; nonhuman; methodology; sensitivity and specificity; metabolism; spectrometry, fluorescence; drug effect; antiinfective agent; chemistry; anti-bacterial agents; evaluation; instrumentation; flavonoid; flavonoids; spectrofluorometry; plant extract; plant extracts; methicillin resistance; staphylococcus aureus; methicillin resistant staphylococcus aureus; thiazoles; bacterium culture; microbiological examination; antibiotic sensitivity; oxidation reduction reaction; oxidation-reduction; concentration response; radiation scattering; 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide; tetrazolium; bacterial viability; tetrazolium salts; antibacterial activity; scattering, radiation; light; thiazole derivative; coloring agents; marchantia convoluta; convoluta; marchantia; antimicrobial susceptibility testing; mtt; resonance light scattering; coloring agent; thiazolyl blue; penicillin resistance; microbial sensitivity tests |
| Journal Title: | Journal of Microbiology and Biotechnology |
| Volume: | 18 |
| Issue: | 1 |
| ISSN: | 1017-7825 |
| Publisher: | The Korean Society for Microbiology and Biotechnology |
| Date Published: | 2008-01-28 |
| Start Page: | 118 |
| End Page: | 123 |
| Language: | English |
| PUBMED: | 18239427 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 3" - "Export Date: 17 November 2011" - "CODEN: JOMBE" - "Source: Scopus" |
