Autoreactivity in human IgG+ memory B cells Journal Article
| Authors: | Tiller, T.; Tsuiji, M.; Yurasov, S.; Velinzon, K.; Nussenzweig, M. C.; Wardemann, H. |
| Article Title: | Autoreactivity in human IgG+ memory B cells |
| Abstract: | More than half of the nascent B cells in humans initially express autoreactive antibodies. However, most of these autoantibodies are removed from the repertoire at two checkpoints before maturation into naive B cells. A third checkpoint excludes remaining autoantibodies from the antigen-experienced IgM+ memory B cell pool. Nevertheless, low-affinity self-reactive antibodies are frequently found in the serum of normal humans. To determine the source of these antibodies, we cloned and expressed antibodies from circulating human IgG+ memory B cells. Surprisingly, we found that self-reactive antibodies including anti-nuclear antibodies were frequently expressed by IgG+ memory B cells in healthy donors. Most of these antibodies were created de novo by somatic hypermutation during the transition between mature naive and IgG+ memory B cells. We conclude that deregulation of self-reactive IgG+ memory B cells may be associated with autoimmunity. © 2007 Elsevier Inc. All rights reserved. |
| Keywords: | controlled study; human cell; polymerase chain reaction; gene expression; immunoreactivity; b-lymphocytes; molecular cloning; somatic hypermutation; immunoglobulin g; autoimmunity; antinuclear antibody; enzyme-linked immunosorbent assay; autoantibody; memory cell; autoantibodies; immunologic memory; antibody production; humdisease; normal human; antibody affinity; cellimmuno; somatic hypermutation, immunoglobulin |
| Journal Title: | Immunity |
| Volume: | 26 |
| Issue: | 2 |
| ISSN: | 1074-7613 |
| Publisher: | Cell Press |
| Date Published: | 2007-02-23 |
| Start Page: | 205 |
| End Page: | 213 |
| Language: | English |
| DOI: | 10.1016/j.immuni.2007.01.009 |
| PUBMED: | 17306569 |
| PROVIDER: | scopus |
| PMCID: | PMC1839941 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 93" - "Export Date: 17 November 2011" - "CODEN: IUNIE" - "Source: Scopus" |
