Reconstitution of S. cerevisiae RNA exosome complexes using recombinantly expressed proteins Journal Article
| Authors: | Zinder, J. C.; Lima, C. D. |
| Article Title: | Reconstitution of S. cerevisiae RNA exosome complexes using recombinantly expressed proteins |
| Abstract: | 3' to 5' RNA degradation is primarily catalyzed by the RNA exosome subunits Dis3 and Rrp6 in the nucleus of Saccharomyces cerevisiae. These enzymes form a complex with the nine-subunit noncatalytic core (Exo9) to carry out their functions in vivo. Protein cofactors Rrp47, Mpp6, and the Mtr4 RNA helicase also assist the complex by modulating its activities and/or recruiting it to specific RNAs for processing or degradation. Here we present our preferred strategy for reconstituting RNA exosomes from S. cerevisiae using purified, recombinantly expressed components. |
| Keywords: | domain; binding; pathways; crystal-structure; nuclear; degradation; budding yeast; exoribonuclease; rrp6; rna decay; rna exosome; paths |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 2062 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2020-01-01 |
| Start Page: | 427 |
| End Page: | 448 |
| Language: | English |
| ACCESSION: | WOS:000651585300022 |
| PROVIDER: | wos |
| PUBMED: | 31768989 |
| DOI: | 10.1007/978-1-4939-9822-7_21 |
| PMCID: | PMC8596991 |
| Notes: | Chapter 21 in "The Eukaryotic RNA Exosome" (ISBN: 978-1-4939-9822-7) -- Source: Wos |
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