Toll receptors remodel epithelia by directing planar-polarized Src and PI3K activity Journal Article


Authors: Tamada, M.; Shi, J.; Bourdot, K. S.; Supriyatno, S.; Palmquist, K. H.; Gutierrez-Ruiz, O. L.; Zallen, J. A.
Article Title: Toll receptors remodel epithelia by directing planar-polarized Src and PI3K activity
Abstract: Toll-like receptors are essential for animal development and survival, with conserved roles in innate immunity, tissue patterning, and cell behavior. The mechanisms by which Toll receptors signal to the nucleus are well characterized, but how Toll receptors generate rapid, localized signals at the cell membrane to produce acute changes in cell polarity and behavior is not known. We show that Drosophila Toll receptors direct epithelial convergent extension by inducing planar-polarized patterns of Src and PI3-kinase (PI3K) activity. Toll receptors target Src activity to specific sites at the membrane, and Src recruits PI3K to the Toll-2 complex through tyrosine phosphorylation of the Toll-2 cytoplasmic domain. Reducing Src or PI3K activity disrupts planar-polarized myosin assembly, cell intercalation, and convergent extension, whereas constitutive Src activity promotes ectopic PI3K and myosin cortical localization. These results demonstrate that Toll receptors direct cell polarity and behavior by locally mobilizing Src and PI3K activity. © 2021 Elsevier Inc.
Keywords: drosophila; toll-like receptor; src; planar polarity; convergent extension; epithelial morphogenesis; pi3-kinase; actomyosin contractility
Journal Title: Developmental Cell
Volume: 56
Issue: 11
ISSN: 1534-5807
Publisher: Cell Press  
Date Published: 2021-06-07
Start Page: 1589
End Page: 1602.e9
Language: English
DOI: 10.1016/j.devcel.2021.04.012
PUBMED: 33932332
PROVIDER: scopus
PMCID: PMC8570296
DOI/URL:
Notes: Article -- Export Date: 1 July 2021 -- Source: Scopus
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