Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin Journal Article
| Authors: | Ortner, V. K.; Sahu, A.; Cordova, M.; Kose, K.; Aleissa, S.; Alessi-Fox, C.; Haedersdal, M.; Rajadhyaksha, M.; Rossi, A. M. |
| Article Title: | Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin |
| Abstract: | We investigated the utility of the fluorescent dye Deep Red Anthraquinone 5 (DRAQ5) for digital staining of optically sectioned skin in comparison to acridine orange (AO). Eight fresh-frozen thawed Mohs discard tissue specimens were stained with AO and DRAQ5, and imaged using an ex vivo confocal microscope at three wavelengths (488 nm and 638 nm for fluorescence, 785 nm for reflectance). Images were overlaid (AO + Reflectance, DRAQ5 + Reflectance), digitally stained, and evaluated by three investigators for perceived image quality (PIQ) and histopathological feature identification. In addition to nuclear staining, AO seemed to stain dermal fibers in a subset of cases in digitally stained images, while DRAQ5 staining was more specific to nuclei. Blinded evaluation showed substantial agreement, favoring DRAQ5 for PIQ (82%, Cl 75%-90%, Gwet's AC 0.74) and for visualization of histopathological features in (81%, Cl 73%-89%, Gwet's AC 0.67), supporting its use in digital staining of multimodal confocal micrographs of skin. © 2020 Wiley-VCH GmbH |
| Keywords: | quality control; confocal microscopy; fluorescence; fluorescent dyes; acridine orange; reflection; ketones; multi-modal; mohs; digital staining; aromatic compounds; tissue specimens; draq5; evcm; tissue fixture; feature identification; frozen-thawed; three-wavelengths |
| Journal Title: | Journal of Biophotonics |
| Volume: | 14 |
| Issue: | 4 |
| ISSN: | 1864-063X |
| Publisher: | Wiley - V C H Verlag GmbH & Co. KGaA |
| Date Published: | 2021-04-01 |
| Start Page: | e202000207 |
| Language: | English |
| DOI: | 10.1002/jbio.202000207 |
| PUBMED: | 33314673 |
| PROVIDER: | scopus |
| PMCID: | PMC8274380 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2021 -- Source: Scopus |
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