Administration of high titer convalescent anti-SARS-CoV-2 plasma: From donor selection to monitoring recipient outcomes Journal Article
| Authors: | Mendoza, R. P.; Fyke, W.; Daniel, D.; Gabutan, E.; Das, B.; Bajaj, H.; Easy, M.; Vasileva, A.; Colbourn, R.; Alawad, M.; Dehghani, A.; Lin, B.; Emechebe, D.; Patel, P.; Jabbar, A.; Nikolov, D. B.; Giovaniello, D.; Kang, S.; Tatem, L.; Bromberg, K.; Augenbraun, M.; Premsrirut, P.; Libien, J.; Norin, A. J. |
| Article Title: | Administration of high titer convalescent anti-SARS-CoV-2 plasma: From donor selection to monitoring recipient outcomes |
| Abstract: | Early in the SARS-CoV-2 pandemic, convalescent plasma (CP) therapy was proposed as a treatment for severely ill patients. We conducted a CP treatment protocol under the Mayo Clinic Extended Access Program at University Hospital Brooklyn (UHB). Potential donors were screened with a lateral flow assay (LFA) for IgM and IgG antibodies against the SARS-CoV-2 S1 receptor-binding domain (RBD). Volunteers that were LFA positive were tested with an ELISA to measure IgG titers against the RBD. Subjects with titers of at least 1:1024 were selected to donate. Most donors with positive LFA had acceptable titers and were eligible to donate. Out of 171 volunteers, only 65 tested positive in the LFA (38.0%), and 55 (32.2%) had titers of at least 1:1024. Before our donation program started, 31 CP units were procured from the New York Blood Center (NYBC). Among the 31 CP units that were obtained from the NYBC, 25 units (80.6%) were positive in the LFA but only 12 units (38.7%) had titers of at least 1:1024. CP was administered to 28 hospitalized COVID-19 patients. Patients who received low titer CP, high titer CP and patients who did not receive CP were followed for 45 days after presentation. Severe adverse events were not associated with CP transfusion. Death was a less frequent outcome for patients that received high titer CP (>1:1024) 38.6% mortality, than patients that received low titer CP (≤1:1024) 77.8% mortality. © 2021 The Authors |
| Keywords: | adult; controlled study; aged; major clinical study; disease course; hypertension; human immunodeficiency virus infection; follow up; heart disease; kidney disease; lung disease; oxygen therapy; retrospective study; enzyme linked immunosorbent assay; coughing; dyspnea; fever; disease severity; virus rna; diabetes mellitus; comorbidity; hospital admission; hydroxychloroquine; immunoassay; glaucoma; vancomycin; corticosteroid; elisa; donor selection; azithromycin; neurologic disease; atrial fibrillation; hyperlipidemia; immunoglobulin g antibody; receptor binding; virus detection; antibody titer; ceftriaxone; artificial ventilation; immunoglobulin m antibody; descriptive research; critically ill patient; recipient; antigen antibody complex; bipolar disorder; microtiter plate assay; tocilizumab; human; male; female; priority journal; article; oxygen desaturation; icu patients; ventilated patient; real time reverse transcription polymerase chain reaction; severe acute respiratory syndrome coronavirus 2; coronavirus disease 2019; covid-19; sars-cov-2; remdesivir; sars-cov-2 antibody; convalescent plasma donation and treatment; lateral flow antibody assay; coronavirus spike glycoprotein; sars-cov-2 convalescent plasma; invasive ventilation; nasopharyngeal swab |
| Journal Title: | Human Immunology |
| Volume: | 82 |
| Issue: | 4 |
| ISSN: | 0198-8859 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2021-04-01 |
| Start Page: | 255 |
| End Page: | 263 |
| Language: | English |
| DOI: | 10.1016/j.humimm.2021.02.007 |
| PUBMED: | 33640208 |
| PROVIDER: | scopus |
| PMCID: | PMC7885628 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 3 May 2021 -- Source: Scopus |
