Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells Journal Article
| Authors: | Lee, G.; Kim, H.; Elkabetz, Y.; Al Shamy, G.; Panagiotakos, G.; Barberi, T.; Tabar, V.; Studer, L. |
| Article Title: | Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells |
| Abstract: | Vertebrate neural crest development depends on pluripotent, migratory precursor cells. Although avian and murine neural crest stem (NCS) cells have been identified, the isolation of human NCS cells has remained elusive. Here we report the derivation of NCS cells from human embryonic stem cells at the neural rosette stage. We show that NCS cells plated at clonal density give rise to multiple neural crest lineages. The human NCS cells can be propagated in vitro and directed toward peripheral nervous system lineages (peripheral neurons, Schwann cells) and mesenchymal lineages (smooth muscle, adipogenic, osteogenic and chondrogenic cells). Transplantation of human NCS cells into the developing chick embryo and adult mouse hosts demonstrates survival, migration and differentiation compatible with neural crest identity. The availability of unlimited numbers of human NCS cells offers new opportunities for studies of neural crest development and for efforts to model and treat neural crest-related disorders. © 2007 Nature Publishing Group. |
| Keywords: | human cell; nonhuman; animal cell; mouse; animals; mice; animal tissue; cell survival; cells, cultured; mesenchyme cell; smooth muscle fiber; embryo; embryonic stem cell; animal experiment; cell differentiation; neurons; in vitro study; cell lineage; vertebrata; aves; cell isolation; murinae; cell migration; cell culture techniques; cell movement; embryonic stem cells; development; rats; neural crest; schwann cell; nerve cell; tissue engineering; cell transplantation; osteoblast; neurology; peripheral nervous system; host; muscle; chick embryo; cells; neural crest cell; transplantation (surgical); crest-related disorders; mesenchymal lineages |
| Journal Title: | Nature Biotechnology |
| Volume: | 25 |
| Issue: | 12 |
| ISSN: | 1087-0156 |
| Publisher: | Nature Publishing Group |
| Date Published: | 2007-12-01 |
| Start Page: | 1468 |
| End Page: | 1475 |
| Language: | English |
| DOI: | 10.1038/nbt1365 |
| PUBMED: | 18037878 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 85" - "Export Date: 17 November 2011" - "CODEN: NABIF" - "Source: Scopus" |
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