Eomes::GFP - A tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo Journal Article

   

Authors:	Kwon, G. S.; Hadjantonakis, A. K.
Article Title:	Eomes::GFP - A tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo
Abstract:	Expression of T-box family member Eomesodermin (Tbr2) is spatiotemporally restricted in the mouse embryo; initially expressed in extraembryonic lineages in the sequential progression from the trophectoderm of the blastocyst, its derivatives the extraembryonic ectoderm, and thereafter the chorion, in addition to the visceral endoderm and primitive streak at gastrula stages, and the telencephalon at later stages. We describe the spatiotemporal expression of GFP in embryos of a Tg(Eomes::GFP) BAC transgenic strain, and have compared it with the localization of endogenous Eomes transcripts and protein. Our analysis reveals the following: (1) robust easily visualized reporter expression in live hemizygous transgenic embryos, (2) increased levels of expression in live homozygous transgenic embryos that are compatible with embryo viability, and (3) a close correlation between endogenous Eomes and GFP reporter expression in BAG transgenic embryos. These features establish the Tg(Eomes::GFP) BAG transgenic strain as a novel reagent for both live imaging and the isolation of Eomes expressing cells from specific locations within the embryo.
Keywords:	protein expression; unclassified drug; nonhuman; pancreas; mouse; animals; mice; animal tissue; embryo; green fluorescent protein; protein; embryo development; diagnostic imaging; central nervous system; bacterial artificial chromosome; transgenic mouse; mice, transgenic; gene expression regulation, developmental; recombinant fusion proteins; limb; gestational age; reporter gene; imaging; green fluorescent proteins; pregnancy; hemizygosity; transgenes; gene location; stomach; live imaging; trophoblast; trophoblasts; mouse embryo; blastocyst; embryo cell; telencephalon; ectoderm; primitive streak; developmental gene; eomesodermin; gastrula; t-box domain proteins; extraembryonic ectoderm; digit condensation; t-box; tbr2; trophectoderm; eomes gene
Journal Title:	Genesis
Volume:	45
Issue:	4
ISSN:	1526-954X
Publisher:	Wiley Blackwell
Date Published:	2007-04-01
Start Page:	208
End Page:	217
Language:	English
DOI:	10.1002/dvg.20293
PUBMED:	17417802
PROVIDER:	scopus
PMCID:	PMC2882854
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-34247577994&partnerID=40&md5=5ac58431c7c66b705b9c6992b2a8483f
Notes:	--- - "Cited By (since 1996): 15" - "Export Date: 17 November 2011" - "CODEN: GNESF" - "Source: Scopus"

https://synapse.mskcc.org/synapse/works/has_related_data_chk/17676