Growth stimulation of human melanocytes: Identification and characterization of melanoma-derived melanocyte growth factor (M-McGF) Journal Article
| Authors: | Ogata, S.; Furuhashi, Y.; Eisinger, M. |
| Article Title: | Growth stimulation of human melanocytes: Identification and characterization of melanoma-derived melanocyte growth factor (M-McGF) |
| Abstract: | The melanocyte growth stimulating factor from human melanoma cells grown in vitro [Eisinger, M. et al, (1985) Science 229,984-986] has been purified and identified as a 14kDa peptide. The activity was shown to be sensitive to acid and heat treatments but resistent to reduction. The factor has strong affinity for heparin-Sepharose and was separated into two fractions by ion-exchange chromatography. Based on its properties, it is grouped in the family of heparin-binding growth factors. Identification of this growth factor will provide a tool for studies of growth regulation of normal and transformed human melanocytes. © 1987. |
| Keywords: | human cell; cell division; melanoma; melanocyte; melanocytes; drug stability; chromatography, gel; electrophoresis, polyacrylamide gel; chromatography, affinity; intermedin; human; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Biochemical and Biophysical Research Communications |
| Volume: | 146 |
| Issue: | 3 |
| ISSN: | 0006-291X |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 1987-08-14 |
| Start Page: | 1204 |
| End Page: | 1211 |
| Language: | English |
| DOI: | 10.1016/0006-291x(87)90776-5 |
| PUBMED: | 3619927 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus; Acknowledgments: We thank Dr. Lloyd for stimulating discussions and critical reading of the manuscript. |
