Growth regulation of human melanocytes: Mitogenic factors in extracts of melanoma, astrocytoma, and fibroblast cell lines Journal Article

  

Authors:	Eisinger, M.; Marko, O.; Ogata, S. I.; Old, L. J.
Article Title:	Growth regulation of human melanocytes: Mitogenic factors in extracts of melanoma, astrocytoma, and fibroblast cell lines
Abstract:	Melanocytes derived from fetal or adult skin do not propagate in vitro unless cultured in the presence of factors such as 12-O-tetradecanoylphorbol 13-acetate (TPA). In a search for physiological factors regulating the growth of melanocytes, extracts of various cultured cell types were tested. Factors produced by melanoma and astrocytoma cell lines support continued proliferation of melanocytes in the absence of TPA. WI-38, a fibroblast cell line derived from human embryonic lung, was the most active source of melanocyte growth factors. No melanocyte growth-promoting activity was found in extracts of cultured neuroblastoma, renal cancer, normal keratinocytes, or renal epithelium. Nerve growth factor, epidermal growth factor, melanocyte-stimulating hormone, transforming growth factor-β, and platelet-derived growth factor did not have growth-promoting activity for melanocytes. The presence of melanocyte growth factors and TPA together resulted in the strongest mitogenic activity for melanocytes, permitting the recovery (at 20 days) of 4 to 20 times as many cells as in growth factor or TPA alone.
Keywords:	human cell; drug efficacy; cell division; melanoma; cell growth; melanocyte; melanocytes; cell line; in vitro study; cell culture; fibroblast; fibroblasts; radioisotope; growth factor; astrocytoma; therapy; thymidine h 3; nervous system; phorbol 13 acetate 12 myristate; tetradecanoylphorbol acetate; growth substances; intermedin; human; priority journal; support, u.s. gov't, p.h.s.; cholera toxin
Journal Title:	Science
Volume:	229
Issue:	4717
ISSN:	0036-8075
Publisher:	American Association for the Advancement of Science
Date Published:	1985-09-06
Start Page:	984
End Page:	986
Language:	English
DOI:	10.1126/science.4023718
PUBMED:	4023718
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022385991&doi=10.1126%2fscience.4023718&partnerID=40&md5=075b4c6961c52a6075425655f5a4ac10
Notes:	Article -- Export Date: 26 October 2021 -- Source: Scopus

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