A marker for neoplastic progression of human melanocytes is a cell surface ectopeptidase Journal Article


Authors: Morrison, M. E.; Vijayasaradhi, S.; Engelstein, D.; Albino, A. P.; Houghton, A. N.
Article Title: A marker for neoplastic progression of human melanocytes is a cell surface ectopeptidase
Abstract: Adenosine deaminase binding protein (ADAbp) is a cell surface glycoprotein that is expressed by normal melanocytes but not by melanoma, the malignant counterpart. ADAbp is specifically downregulated during malignant transformation of melanocytes. Recently, we have developed a system that progressively transforms melanocytes in vitro in defined steps. Transduction with v-Ha-ras oncogene followed by long-term culture leads to a cell phenotype and genotype that specifically mimics human melanoma. Loss of ADAbp expression occurred concomitantly with the emergence of growth factor independence and appearance of specific chromosomal abnormalities. The cellular function of ADAbp has not been defined. To characterize ADAbp, the mature 110- kD form was purified from human kidney. Five tryptic peptides from purified human ADAbp revealed 100% homology to a serine protease, human dipeptidyl peptidase IV (DPP IV), also known as CD26. DPP IV activity was detected in lysates from human melanocytes and renal carcinoma cells but not melanoma cells, and DPP IV activity could be specifically isolated from melanocytes by binding to ADA or to $27 monoclonal antibody against ADAbp. These findings show that ADAbp is a cell surface ectopeptidase that is tightly regulated during neoplastic transformation of melanocytes. © 1993, Rockefeller University Press., All rights reserved.
Keywords: controlled study; human cell; protein analysis; animal; melanoma; melanocyte; melanocytes; tumor markers, biological; tumor cells, cultured; enzyme activity; oncogene h ras; genetic transduction; cell transformation, neoplastic; amino acid sequence; molecular sequence data; sequence homology, amino acid; cell surface marker; carrier proteins; cell membrane; binding protein; genes, ras; sequence homology; malignant transformation; dipeptidyl peptidase iv; adenosine deaminase; human; priority journal; article; antigens, cd26; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; radioimmunoprecipitation assay; dipeptidyl peptidases
Journal Title: Journal of Experimental Medicine
Volume: 177
Issue: 4
ISSN: 0022-1007
Publisher: Rockefeller University Press  
Date Published: 1993-04-01
Start Page: 1135
End Page: 1143
Language: English
DOI: 10.1084/jem.177.4.1135
PUBMED: 8096237
PROVIDER: scopus
PMCID: PMC2190962
DOI/URL:
Notes: Source: Scopus
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  1. Alan N Houghton
    364 Houghton
  2. Anthony P. Albino
    111 Albino