Expression and localization of aminopeptidase A, aminopeptidase N, and dipeptidyl peptidase IV in benign and malignant human prostate tissue Journal Article


Authors: Bogenrieder, T.; Finstad, C. L.; Freeman, R. H.; Papandreou, C. N.; Scher, H. I.; Albino, A. P.; Reuter, V. E.; Nanus, D. M.
Article Title: Expression and localization of aminopeptidase A, aminopeptidase N, and dipeptidyl peptidase IV in benign and malignant human prostate tissue
Abstract: BACKGROUND. Cell-surface peptidases are ectoenzymes which regulate the access of bioactive peptides to their receptors on cell membranes. Abnormalities in their expression and function result in altered peptide activity which contribute to neoplastic transformation and/or progression. METHODS. Expression of aminopeptidase A (APA), aminopeptidase N (APN, CD13), and dipeptidyl peptidase IV (DPP IV, CD26) was immunohistochemically examined in 20 benign and 33 malignant prostate tissues (19 primaries and 14 metastases). RESULTS. Benign prostatic stroma exhibited no APA, APN, or DPP IV immunoreactivity. Stromal cells surrounding prostatic carcinoma cells demonstrated increased APA expression in 24/33 (73%) of tumors. Benign prostatic epithelial cells strongly expressed APN and DPP IV but not APA. In contrast, APN was expressed in >80% of tumor cells in 5/33 (15%) of specimens, heterogeneously expressed (20-80% of cells positive) in 4/33 (12%) of specimens, and minimally expressed or absent in 24/33 (73%) of tumor specimens, with a similar pattern of expression in primary and metastatic tumors. DPP IV was expressed by >80% of tumor cells in 18/19 (95%) of primary prostate cancer specimens, but in only 7/14 (50%) of metastases. CONCLUSIONS. These data show that cell-surface peptidases are differentially expressed by normal prostatic stromal and epithelial cells, with increased expreSSion of APA in the stroma surrounding prostate cancer cells, absent APN expression in most tumor cells, and a decreased frequency of DPP IV expression in metastatic tumors. Further studies will elucidate the biological effects of the presence or loss of cell-surface peptidases in the benign and malignant prostate.
Keywords: immunohistochemistry; adult; clinical article; human tissue; protein expression; aged; aged, 80 and over; bone neoplasms; middle aged; liver neoplasms; lymph nodes; phenotype; lung neoplasms; biopsy; monoclonal antibody; prostate cancer; prostatic neoplasms; lung metastasis; antibodies, monoclonal; prostate; cancer cell; epithelium cell; epithelium; stroma cell; malignant transformation; enzyme localization; soft tissue neoplasms; prostate disease; microsomal aminopeptidase; dipeptidyl peptidase iv; antigens, cd13; humans; human; male; priority journal; article; antigens, cd26; aminopeptidases; glutamyl aminopeptidase
Journal Title: Prostate
Volume: 33
Issue: 4
ISSN: 0270-4137
Publisher: John Wiley & Sons  
Date Published: 1997-12-01
Start Page: 225
End Page: 232
Language: English
DOI: 10.1002/(sici)1097-0045(19971201)33:4<225::aid-pros1>3.0.co;2-g
PUBMED: 9397193
PROVIDER: scopus
DOI/URL:
Notes: Source: Scopus
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MSK Authors
  1. Victor Reuter
    1084 Reuter
  2. Howard Scher
    991 Scher
  3. David M. Nanus
    61 Nanus
  4. Anthony P. Albino
    94 Albino
  5. Connie L. Finstad
    37 Finstad
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