Aminopeptidase A expression and enzymatic activity in primary human renal cancers Journal Article

  


Authors: Nanus, D. M.; Bogenrieder, T.; Papandreou, C. N.; Finstad, C. L.; Lee, A.; Vlamis, V.; Motzer, R. J.; Bander, N. H.; Albino, A. P.; Reuter, V. E.
Article Title: Aminopeptidase A expression and enzymatic activity in primary human renal cancers
Abstract: The gp160 human kidney differentiation antigen is identical to human aminopeptidase A (APA), a zinc-dependent cell-surface metallopeptidase which hydrolyzes peptides with N-terminal acidic residues. GP160/APA is constitutively expressed by proximal tubule cells, the normal cellular counterpart of most renal cancers (RCs). Immunohistochemical analysis of gp160/APA protein expression in 62 primary renal tumor specimens using monoclonal antibody S4 revealed heterogeneous or homogeneous expression of gp160/APA in 46/51 (90%) of clear cell carcinomas in contrast with 1/8 (13%) papillary renal tumors and 0/3 oncocytomas (p<0.001). Analysis of five primary clear cell carcinomas for gp160 protein expression immunohistochemically and associated APA catalytic activity revealed one tumor which expressed gp160/APA protein which was enzymatically inactive. Direct sequence analysis of DNA derived from this specimen could not detect mutations within the zinc-binding domain which would eliminate gp160/APA catalytic activity. These data indicate that the gp160/APA protein is expressed by primary clear cell but not papillary RCs or oncocytomas, and that alterations in gp160/APA protein including loss of protein expression or enzymatic activity occur in ~20% of primary clear cell RCs.
Keywords: immunohistochemistry; human tissue; protein expression; human cell; sequence analysis; gene deletion; histopathology; cancer staging; carcinoma, papillary; neoplasm proteins; tumor cells, cultured; enzyme activity; oncocytoma; adenoma, oxyphilic; kidney neoplasms; kidney; amino acid sequence; molecular sequence data; tissue distribution; rna, messenger; dna, neoplasm; base sequence; clear cell carcinoma; kidney cancer; papillary carcinoma; differentiation antigen; renal cancer; aminopeptidase; humans; human; priority journal; article; aminopeptidases; angiotensins; cell-surface peptidase; glutamyl aminopeptidase
Journal Title: International Journal of Oncology
Volume: 13
Issue: 2
ISSN: 1019-6439
Publisher: Spandidos Publications  
Date Published: 1998-08-01
Start Page: 261
End Page: 267
Language: English
PUBMED: 9664120
PROVIDER: scopus
DOI: 10.3892/ijo.13.2.261
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-13144305028&partnerID=40&md5=04ab28a8ca3ca91a557a0aa7ccc0a013
Notes: Source: Scopus
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MSK Authors
  1. Robert Motzer
    1243 Motzer
  2. Victor Reuter
    1223 Reuter
  3. Christos N Papandreou
    20 Papandreou
  4. Vaia Vlamis
    38 Vlamis
  5. David M. Nanus
    66 Nanus
  6. Connie L. Finstad
    45 Finstad
  7. Thomas Bogenrieder
    18 Bogenrieder
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