Molecular cloning of the human kidney differentiation antigen gp160: Human aminopeptidase A Journal Article

   


Authors: Nanus, D. M.; Engelstein, D.; Gastl, G. A.; Gluck, L.; Vidal, M. J.; Morrison, M.; Finstad, C. L.; Bander, N. H.; Albino, A. P.
Article Title: Molecular cloning of the human kidney differentiation antigen gp160: Human aminopeptidase A
Abstract: gp160 is a cell surface differentiation-related glycoprotein of 160 kDa expressed by epithelial cells of the glomerulus and proximal tubule cells of the human nephron but only by a subset of renal cell carcinomas (RCCs). We have reported that gp160 expression correlates with the resistance of cultured RCCs to the antiproliferative effects of α interferon, while lack of expression correlates with sensitivity to α interferon. In this study, we have purified gp160 protein, obtained partial sequences of random peptides, and isolated a full-length cDNA. The gp160 cDNA possesses 78% homology to the murine BP-1/6C3 antigen, a B-lymphocyte differentiation protein that exhibits aminopeptidase A (APA; EC 3.4.11.7) activity. Enzymatic assays on human RCC cell lines indicated a 100% concordance between APA activity and gp160 expression. APA activity of gp160-expressing RCC cells was increased or decreased by a panel of APA activators or inhibitors, respectively. Furthermore, anti-gp160 monoclonal antibodies immunoprecipitate APA activity from RCC cell lysates and selectively deplete APA activity from RCC cell extracts. These data indicate that the gp160 human kidney/RCC glycoprotein is human APA.
Keywords: human cell; alpha interferon; antigen expression; protein analysis; cell differentiation; kidney carcinoma; amino acid sequence; enzyme analysis; murinae; dna sequence; sequence homology; differentiation antigen; kidney cell; kidney proximal tubule; renal cancer; nephron; aminopeptidase; glomerulus; cdna cloning; human; priority journal; article; angiotensins; glycoprotein gp 160; ectopeptidases
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 90
Issue: 15
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1993-08-01
Start Page: 7069
End Page: 7073
Language: English
DOI: 10.1073/pnas.90.15.7069
PUBMED: 8346219
PROVIDER: scopus
PMCID: PMC47077
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0027327945&doi=10.1073%2fpnas.90.15.7069&partnerID=40&md5=d35a8f174d596918298a00179133e605
Notes: Article -- Export Date: 1 March 2019 -- Source: Scopus
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MSK Authors
  1. Neil Harrison Bander
    62 Bander
  2. David M. Nanus
    66 Nanus
  3. Anthony P. Albino
    111 Albino
  4. Connie L. Finstad
    45 Finstad
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