Abl and Canoe/Afadin mediate mechanotransduction at tricellular junctions Journal Article
| Authors: | Yu, H. H.; Zallen, J. A. |
| Article Title: | Abl and Canoe/Afadin mediate mechanotransduction at tricellular junctions |
| Abstract: | Epithelial structure is generated by the dynamic reorganization of cells in response to mechanical forces. Adherens junctions transmit forces between cells, but how cells sense and respond to these forces in vivo is not well understood. We identify a mechanotransduction pathway involving the Abl tyrosine kinase and Canoe/Afadin that stabilizes cell adhesion under tension at tricellular junctions in the Drosophila embryo. Canoe is recruited to tricellular junctions in response to actomyosin contractility, and this mechanosensitivity requires Abl-dependent phosphorylation of a conserved tyrosine in the Canoe actin-binding domain. Preventing Canoe tyrosine phosphorylation destabilizes tricellular adhesion, and anchoring Canoe at tricellular junctions independently of mechanical inputs aberrantly stabilizes adhesion, arresting cell rearrangement. These results identify a force-responsive mechanism that stabilizes tricellular adhesion under tension during epithelial remodeling. © 2020 American Association for the Advancement of Science. All rights reserved. |
| Keywords: | amino acid; fly; biochemistry; adhesion; chemical binding; biomechanics; cell component |
| Journal Title: | Science |
| Volume: | 370 |
| Issue: | 6520 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 2020-11-27 |
| Start Page: | eaba5528 |
| Language: | English |
| DOI: | 10.1126/science.aba5528 |
| PUBMED: | 33243859 |
| PROVIDER: | scopus |
| PMCID: | PMC8559527 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 4 January 2021 -- Source: Scopus |
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