DGCR8-dependent microRNA biogenesis is essential for skin development Journal Article
| Authors: | Yi, R.; Pasolli, H. A.; Landthaler, M.; Hafner, M.; Ojo, T.; Sheridan, R.; Sander, C.; O'Carroll, D.; Stoffel, M.; Tuschl, T.; Fuchs, E. |
| Article Title: | DGCR8-dependent microRNA biogenesis is essential for skin development |
| Abstract: | MicroRNAs play important roles in animal development. Numerous conditional knockout (cKO) studies of Dicer have been performed to interrogate the functions of microRNA during mammalian development. However, because Dicer was recently implicated in the biogenesis of endogenous siRNAs in mammals, it raises the question whether the Dicer cKO defects can be attributable to the loss of microRNAs. Previously, we and others conditionally targeted Dicer and identified its critical roles in embryonic skin morphogenesis. Here, we focus explicitly on microRNAs by taking a parallel strategy with Dgcr8, encoding an essential component of the microprocessor complex that is exclusively required for microRNA biogenesis. With this comparative analysis, we show definitively that the Dicer- and Dgcr8-null skin defects are both striking and indistinguishable. By deep sequencing analysis of microRNA depletion in both Dicer- and Dgcr8-null skin, we demonstrate that most abundantly expressed skin microRNAs are dependent on both Dicer and DGCR8. Our results underscore a specific importance of microRNAs in controlling mammalian skin development. © 2008 by The National Academy of Sciences of the USA. |
| Keywords: | controlled study; unclassified drug; sequence analysis; nonhuman; comparative study; proteins; animal cell; mouse; phenotype; mammalia; animals; mice; mice, knockout; animal tissue; microrna; skin defect; protein; animalia; skin; dead-box rna helicases; newborn; micrornas; biogenesis; dicer; organogenesis; small rnas; protein dgcr8; rna degradation; endoribonucleases; skin abnormalities |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 106 |
| Issue: | 2 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2009-01-13 |
| Start Page: | 498 |
| End Page: | 502 |
| Language: | English |
| DOI: | 10.1073/pnas.0810766105 |
| PUBMED: | 19114655 |
| PROVIDER: | scopus |
| PMCID: | PMC2626731 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 16" - "Export Date: 30 November 2010" - "CODEN: PNASA" - "Source: Scopus" |
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