Canonical MicroRNA activity facilitates but may be dispensable for transcription factor-mediated reprogramming Journal Article

   


Authors: Liu, Z.; Skamagki, M.; Kim, K.; Zhao, R.
Article Title: Canonical MicroRNA activity facilitates but may be dispensable for transcription factor-mediated reprogramming
Abstract: MicroRNAs (miRNAs) are important regulators of reprogramming of somatic cells into induced pluripotent stem cells (iPSCs); however, it is unclear whether miRNAs are required for reprogramming and whether miRNA activity as a whole facilitates reprogramming. Here we report on successful reprogramming of mouse fibroblasts and neural stem cells (NSCs) lacking Dgcr8, a factor required for the biogenesis of canonical miRNAs, by Yamanaka factors, albeit at decreased efficiencies. Though iPSCs derived from Dgcr8-deficient mouse fibroblasts or NSCs were able to self-renew and expressed pluripotency-associated markers, they exhibited poor differentiation potential into mature somatic tissues, similar to Dgcr8-/- embryonic stem cells. The differentiation defects could be rescued with expression of DGCR8 cDNA. Our data demonstrate that while miRNA activity as a whole facilitates reprogramming, canonical miRNA may be dispensable in the derivation of iPSCs. © 2015 The Authors.
Keywords: controlled study; nonhuman; animal cell; mouse; animal tissue; microrna; embryo; embryonic stem cell; animal experiment; genotype; transcription factor; cell renewal; neural stem cell; cell differentiation; alkaline phosphatase; fibroblast; karyotyping; tissue differentiation; transcription factor nanog; complementary dna; biogenesis; nuclear reprogramming; microrna 9; limit of detection; stage specific embryo antigen 1; microrna 20a; priority journal; article
Journal Title: Stem Cell Reports
Volume: 5
Issue: 6
ISSN: 2213-6711
Publisher: Cell Press  
Date Published: 2015-12-08
Start Page: 1119
End Page: 1127
Language: English
DOI: 10.1016/j.stemcr.2015.11.002
PROVIDER: scopus
PUBMED: 26651605
PMCID: PMC4682342
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84949518715&partnerID=40&md5=d4d8f4cef857336496715b0fb1460cb8
Notes: Article -- Export Date: 7 January 2016 -- 1119 -- Source: Scopus
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MSK Authors
  1. Kitai Kim
    13 Kim
  2. Maria Skamagki
    6 Skamagki
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